Junaid O Q, Wong K T, Khaw L T, Mahmud R, Vythilingam I
Department of Parasitology, Faculty of Medicine, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia.
Department of Biological Science, Faculty of Science, Federal University Kashere, PMB 0182, Gombe State, Nigeria.
Trop Biomed. 2018 Dec 1;35(4):981-998.
Co-infection with multiple different parasites is a common phenomenon in both human and animals. Among parasites that frequently co-infect the same hosts, are the filarial worms and malaria parasites. Despite this, the mechanisms underlying the interactions between these parasites is still relatively unexplored with very few studies available on the resulting pathologies due to co-infection by filarial nematodes and malaria parasites. Hence, this study investigated the histopathological effect of Brugia pahangi and Plasmodium berghei ANKA (PbA) infections in gerbil host. Gerbils grouped into B. pahangi-infected, PbA-infected, B. pahangi and PbA-coinfected, and uninfected control, were necropsied at different time points of post PbA infections. Brugia pahangi infections in the gerbils were first initiated by subcutaneous inoculation of 50 infective larvae, while PbA infections were done by intraperitoneal injection of 10 parasitized red blood cells after 70 days patent period of B. pahangi. Organs such as the lungs, kidneys, spleen, heart and liver were harvested aseptically at the point of necropsy. There was significant hepatosplenomegaly observed in both PbA-infected only and coinfected gerbils. The spleen, liver and lungs were heavily pigmented. Both B. pahangi and PbA infections (mono and coinfections) resulted in pulmonary edema, while glomerulonephritis was associated with PbA infections. The presence of both parasites induced extramedullary hematopoiesis in the spleen and liver. These findings suggest that the pathologies associated with coinfected gerbils were synergistically induced by both B. pahangi and PbA infections.
多种不同寄生虫的共感染在人类和动物中都是常见现象。在经常共同感染同一宿主的寄生虫中，有丝虫和疟原虫。尽管如此，这些寄生虫之间相互作用的潜在机制仍相对未被探索，关于丝虫线虫和疟原虫共感染导致的病理学研究非常少。因此，本研究调查了彭亨布鲁线虫（Brugia pahangi）和伯氏疟原虫安卡拉株（PbA）感染对沙鼠宿主的组织病理学影响。将沙鼠分为感染彭亨布鲁线虫组、感染PbA组、同时感染彭亨布鲁线虫和PbA组以及未感染对照组，在感染PbA后的不同时间点进行剖检。沙鼠的彭亨布鲁线虫感染首先通过皮下接种50条感染性幼虫启动，而在彭亨布鲁线虫有70天的潜伏期后，通过腹腔注射10个寄生有疟原虫的红细胞来进行PbA感染。在剖检时无菌采集肺、肾、脾、心脏和肝脏等器官。仅感染PbA的沙鼠和共感染的沙鼠均观察到明显的肝脾肿大。脾、肝和肺有大量色素沉着。彭亨布鲁线虫和PbA感染（单一感染和共感染）均导致肺水肿，而肾小球肾炎与PbA感染有关。两种寄生虫的存在均诱导脾和肝出现髓外造血。这些发现表明，与共感染沙鼠相关的病理学变化是由彭亨布鲁线虫和PbA感染协同诱导的。
