多位点基因分析揭示了在呈现扁平茎和叶变症状的植株中存在两个植原体组。

Multilocus gene analysis reveals the presence of two phytoplasma groups in showing flat stem and phyllody.

作者信息

Panda Priyam, Nigam Amrita, Rao G P

机构信息

Discipline of Life Sciences, Indira Gandhi National Open University, New Delhi, 110068 India.

Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.

出版信息

3 Biotech. 2021 Mar;11(3):122. doi: 10.1007/s13205-021-02666-2. Epub 2021 Feb 11.

DOI:10.1007/s13205-021-02666-2

PMID:33633922

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7878611/

Abstract

UNLABELLED

Rose balsam () is an important ornamental species grown worldwide for its attractive flowers and also having medicinal properties. Flat stem, little leaf, and phyllody symptoms were observed in nurseries in Uttar Pradesh and Tripura states of India during surveys from 2018 to 2020, with an incidence from 6 to 27%. Amplicons of ~ 1.2 kb were amplified in all the tested symptomatic samples of using universal phytoplasma primer pairs from different surveyed locations, but not from the asymptomatic plants. Pairwise sequence comparison, phylogeny, and virtual RFLP analysis of 16S rRNA gene sequences identified the phytoplasmas as 16SrI-B subgroup strain from Tripura (Lembucherra) and 16SrII-D subgroup strain from Uttar Pradesh (Gorakhpur and Faizabad). Phytoplasma presence and identity was further confirmed by amplifying , , and genes. This is the first report of 16SrI-B and 16SrII-D phytoplasmas detection in in the world.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-021-02666-2.

摘要

未标注

玫瑰香脂（）是一种重要的观赏植物，因其迷人的花朵而在全球种植，同时还具有药用特性。在2018年至2020年的调查期间，在印度北方邦和特里普拉邦的苗圃中观察到扁平茎、小叶和叶变症状，发病率为6%至27%。使用来自不同调查地点的通用植原体引物对，在所有测试的有症状样本中扩增出了约1.2 kb的扩增子，但无症状植物中未扩增出。对16S rRNA基因序列进行成对序列比较、系统发育分析和虚拟RFLP分析，确定这些植原体为来自特里普拉邦（莱姆布彻拉）的16SrI-B亚组菌株和来自北方邦（戈勒克布尔和法扎巴德）的16SrII-D亚组菌株。通过扩增、、和基因，进一步证实了植原体的存在和身份。这是世界上首次在中检测到16SrI-B和16SrII-D植原体的报告。