Institute of Human Genetics, University of Leipzig Medical Center, Leipzig, Germany.
Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, Leipzig, Germany.
Methods Mol Biol. 2021;2269:167-174. doi: 10.1007/978-1-0716-1225-5_12.
Three-dimensional (3D) cell cultures combining multipotent mesenchymal stromal cells (MSC), tendon extracellular matrix scaffolds, and mechanical stimulation by a bioreactor have been used to induce tenogenic differentiation in vitro. Yet, these conditions alone do not mimic the environment of acute inflammatory tendon disease adequately, thus the results of such studies are not representatives for tendon regeneration after acute injury. In this chapter, we describe two different approaches to introduce inflammatory stimuli, comprising co-culture with leukocytes and supplementation with the cytokines IL-1 β and TNF-α. The presented in vitro model of inflammatory tendon disease could be used to study musculoskeletal pathophysiology and regeneration in more depth.
三维（3D）细胞培养物结合多能间充质基质细胞（MSC）、肌腱细胞外基质支架和生物反应器的机械刺激已被用于体外诱导肌腱发生分化。然而，这些条件本身并不能充分模拟急性炎症性肌腱疾病的环境，因此这些研究的结果不能代表急性损伤后肌腱的再生。在本章中，我们描述了两种引入炎症刺激的不同方法，包括与白细胞共培养和补充白细胞介素-1β和肿瘤坏死因子-α。所提出的炎症性肌腱疾病的体外模型可用于更深入地研究肌肉骨骼的病理生理学和再生。
