Department of Plant Agriculture, Ontario Agriculture College, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada.
Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, Iran.
Molecules. 2021 Mar 5;26(5):1395. doi: 10.3390/molecules26051395.
Efforts to develop the necessary biotechnologies in Greater Celandine ( L.), a leading plant resource for the development of plant-derived medicines, have been hampered by the lack of knowledge about transcriptome and metabolome regulations of its medicinal components. Therefore, this study aimed to examine the effect of abiotic elicitors, methyl jasmonate (MJ) and salicylic acid (SA), at different time courses (12, 24, 48, and 72 h), on expression and metabolome of key benzophenanthridine alkaloids (BPAs) in an optimized in vitro culture. Gene expression analysis indicated the upregulation of (cheilanthifoline synthase) to 2.62, 4.85, and 7.28 times higher than the control at 12, 24, and 48 h respectively, under MJ elicitation. Besides, MJ upregulated the expression of (tetrahydroprotoberberine -methyltransferase) to 2.79, 4.75, and 7.21 times at 12, 24, and 48 h respectively, compared to the control. Investigation of BPAs revealed a significant enhancement in the chelidonine content (9.86 µg/mg) after 72 h of MJ elicitation. Additionally, sanguinarine content increased to its highest level (3.42 µg/mg) after 24 h of MJ elicitation; however, no significant enhancement was detected in its content in shorter elicitation time courses. Generally, higher gene expression and BPAs' level was observed through longer elicitation courses (48 and 72 h). Our findings take part in improving the understanding of transcription and metabolic regulation of BPAs in cultured Greater Celandine cells.
为了开发大飞燕草（Chelidonium majus）这种植物源性药物的主要植物资源，人们一直致力于开发必要的生物技术，但由于缺乏有关其药用成分的转录组和代谢组调控的知识，这一目标受到了阻碍。因此，本研究旨在探讨不同时间（12、24、48 和 72 小时）下非生物诱导子茉莉酸甲酯（MJ）和水杨酸（SA）对优化体外培养中关键苯并菲啶生物碱（BPAs）表达和代谢组的影响。基因表达分析表明，在 MJ 诱导下，与对照相比，12、24 和 48 小时时（ cheilanthifoline 合酶）的表达分别上调至 2.62、4.85 和 7.28 倍。此外，与对照相比，MJ 分别在 12、24 和 48 小时时将（四氢原小檗碱甲基转移酶）的表达上调至 2.79、4.75 和 7.21 倍。对 BPAs 的研究发现，MJ 诱导 72 小时后，刻叶紫堇碱含量（9.86 µg/mg）显著增加。此外，血根碱含量在 MJ 诱导 24 小时后达到最高水平（3.42 µg/mg）；然而，在较短的诱导时间过程中，其含量没有显著增加。一般来说，较长的诱导时间（48 和 72 小时）会观察到更高的基因表达和 BPAs 水平。我们的研究结果有助于提高对培养大飞燕草细胞中 BPAs 转录和代谢调控的理解。
