农业管理措施影响茶园土壤酶活性和细菌群落结构。

Agricultural management practices influence the soil enzyme activity and bacterial community structure in tea plantations.

作者信息

Chen Yu-Pei, Tsai Chia-Fang, Rekha P D, Ghate Sudeep D, Huang Hsi-Yuan, Hsu Yi-Han, Liaw Li-Ling, Young Chiu-Chung

机构信息

Department of Public Health and Medical Technology, Xiamen Medical College, Xiamen, 361023, Fujian, China.

Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College, Xiamen, 361023, Fujian, China.

出版信息

Bot Stud. 2021 May 18;62(1):8. doi: 10.1186/s40529-021-00314-9.

DOI:10.1186/s40529-021-00314-9

PMID:34003387

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8131499/

Abstract

BACKGROUND

The soil quality and health of the tea plantations are dependent on agriculture management practices, and long-term chemical fertilizer use is implicated in soil decline. Hence, several sustainable practices are used to improve and maintain the soil quality. Here, in this study, changes in soil properties, enzymatic activity, and dysbiosis in bacterial community composition were compared using three agricultural management practices, namely conventional (CA), sustainable (SA), and transformational agriculture (TA) in the tea plantation during 2016 and 2017 period. Soil samples at two-months intervals were collected and analyzed.

RESULTS

The results of the enzyme activities revealed that acid phosphatase, arylsulfatase, β-glucosidase, and urease activities differed considerably among the soils representing the three management practices. Combining the redundancy and multiple regression analysis, the change in the arylsulfatase activity was explained by soil pH as a significant predictor in the SA soils. The soil bacterial community was predominated by the phyla Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, and Bacteroidetes in the soil throughout the sampling period. Higher Alpha diversity scores indicated increased bacterial abundance and diversity in the SA soils. A significant relationship between bacterial richness indices (SOBS, Chao and ACE) and soil pH, K and, P was observed in the SA soils. The diversity indices namely Shannon and Simpson also showed variations, suggesting the shift in the diversity of less abundant and more common species. Furthermore, the agricultural management practices, soil pH fluctuation, and the extractable elements had a greater influence on bacterial structure than that of temporal change.

CONCLUSIONS

Based on the cross-over analysis of the bacterial composition, enzymatic activity, and soil properties, the relationship between bacterial composition and biologically-driven ecological processes can be identified as indicators of sustainability for the tea plantation.

摘要

背景

茶园的土壤质量和健康状况取决于农业管理措施，长期使用化肥会导致土壤质量下降。因此，人们采用了几种可持续措施来改善和维持土壤质量。在本研究中，于2016年至2017年期间，在茶园采用常规农业（CA）、可持续农业（SA）和转型农业（TA）这三种农业管理措施，比较了土壤性质、酶活性以及细菌群落组成失调的变化情况。每隔两个月采集土壤样本并进行分析。

结果

酶活性结果显示，代表三种管理措施的土壤中，酸性磷酸酶、芳基硫酸酯酶、β-葡萄糖苷酶和脲酶的活性存在显著差异。结合冗余分析和多元回归分析，在可持续农业土壤中，土壤pH是芳基硫酸酯酶活性变化的重要预测指标。在整个采样期间，土壤细菌群落主要由变形菌门、酸杆菌门、放线菌门、绿弯菌门和拟杆菌门组成。较高的α多样性指数表明可持续农业土壤中细菌丰度和多样性增加。在可持续农业土壤中，观察到细菌丰富度指数（单样本观测数、Chao和ACE）与土壤pH、钾和磷之间存在显著关系。香农指数和辛普森指数等多样性指数也显示出变化，表明稀有物种和常见物种的多样性发生了变化。此外，农业管理措施、土壤pH波动和可提取元素对细菌结构的影响大于时间变化。