Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science, Guangxi Normal University, Guilin, China.
School of Mechanical and Electrical Engineering, Guilin University of Electronic Technology, Guilin, China.
BMC Plant Biol. 2022 Jan 7;22(1):21. doi: 10.1186/s12870-021-03408-5.
Brassica napus is an important agricultural species, improving stress resistance was one of the main breeding goals at present. Non-specific lipid transfer proteins (nsLTPs) are small, basic proteins which are involved in some biotic or abiotic stress responses. B. napus is susceptible to a variety of fungal diseases, so identify the BnLTPs and their expression in disease responses is very important. The common reference genome of B. napus does not contain all B. napus genes because of gene presence/absence variations between individuals. Therefore, it was necessary to search for candidate BnLTP genes in the B. napus pangenome.
In the present study, the BnLTP genes were identified throughout the pangenome, and different BnLTP genes were presented among varieties. Totally, 246 BnLTP genes were identified and could be divided into five types (1, 2, C, D, and G). The classification, phylogenetic reconstruction, chromosome distribution, functional annotation, and gene expression were analyzed. We also identified potential cis-elements that respond to biotic and abiotic stresses in the 2 kb upstream regions of all BnLTP genes. RNA sequencing analysis showed that the BnLTP genes were involved in the response to Sclerotinia sclerotiorum infection. We identified 32 BnLTPs linked to blackleg resistance quantitative trait locus (QTL).
The identification and analysis of LTP genes in the B. napus pangenome could help to elucidate the function of BnLTP family members and provide new information for future molecular breeding in B. napus.
油菜是一种重要的农业物种，提高其抗逆性是目前的主要育种目标之一。非特异性脂质转移蛋白（nsLTPs）是一种小而碱性的蛋白质，参与一些生物或非生物胁迫反应。油菜易受多种真菌病害的影响，因此鉴定 BnLTPs 及其在疾病反应中的表达非常重要。由于个体之间基因的存在/缺失变异，油菜的常见参考基因组并不包含所有油菜基因。因此，有必要在油菜泛基因组中搜索候选 BnLTP 基因。
本研究在油菜泛基因组中鉴定了 BnLTP 基因，不同品种中存在不同的 BnLTP 基因。共鉴定出 246 个 BnLTP 基因，可分为 5 种类型（1、2、C、D 和 G）。对其进行了分类、系统发育重建、染色体分布、功能注释和基因表达分析。我们还鉴定了所有 BnLTP 基因 2kb 上游区域中潜在的响应生物和非生物胁迫的顺式元件。RNA 测序分析表明，BnLTP 基因参与了对核盘菌感染的反应。我们鉴定了 32 个与黑胫病抗性数量性状位点（QTL）相关的 BnLTP。
油菜泛基因组中 LTP 基因的鉴定和分析有助于阐明 BnLTP 家族成员的功能，并为油菜的未来分子育种提供新的信息。
