This study attempted to remove acrylonitrile and acetophenone from simulated acrylonitrile butadiene styrene (ABS) based wastewater while recovering nitrogen and phosphorus using the carbohydrate-rich filamentous microalgae Tribonema sp.. Results showed that typical acetophenone and acrylonitrile presented significant inhibitory effect on Tribonema sp. growth and co-metabolism of CO improved the tolerance of Tribonema sp. to toxic pollutants. The microalgae biomass increased by 34.47% (3.16 g/L) and 58.17% (3.97 g/L) via supplementing 2% CO in the 100 mg/L acrylonitrile and acetophenone groups, respectively. The filamentous microalga was rich in carbohydrates and its productivity was further enhanced by 32.52% and 70.34%, respectively, in 100 mg/L acrylonitrile and acetophenone groups with 2% CO supplement. The synergistic CO supply strategy effectively enhanced the biomass production of filamentous microalgae, and moreover, improved the treatment efficiency of ABS based wastewater simulated by acetophenone or acrylonitrile addition, while at same time enhanced the recovery of nitrogen and phosphorus nutrients.