[中国宁夏南部生态移民迁出区不同恢复模式下土壤微生物群落特征]
[Characteristics of soil microbial communities in different restoration models in the ecological immigrants' emigration area in southern Ningxia, China].
作者信息
Yang Hu, Ma Qiao-Rong, Yang Jun-Long, Zhou Liang, Cao Bing, Zhang Wei-Jiang
机构信息
School of Agriculture, Ningxia University, Yinchuan 750021, China.
School of Civil and Hydraulic Engineering, Ningxia University, Yinchuan 750021, China.
出版信息
Ying Yong Sheng Tai Xue Bao. 2022 Jan;33(1):219-228. doi: 10.13287/j.1001-9332.202201.036.
To reveal the effects of plantations on soil microbial environment,the composition and diversity of soil fungi and bacterial communities in five restoration models (, , , , natural restoration) in the mountainous area of southern Ningxia were compared by using high-throughput sequencing technology. The correlation between soil physical-chemical properties and dominant microbial groups was analyzed. The results showed that: 1) Dominant fungi in different restoration models were Ascomycota, Basidiomycota, Mortierellomycota, and unclassified fungi, which accounted for 90% of total fungal community. The dominant soil bacteria were Actinobacteria, Proteobacteria, Acidobacteriota, Chloroflexi, and other bacteria, accounting for more than 80% of total bacterial community. 2) The diversity of soil fungi in forest was the highest, with Shannon index, and Simpson index being 3.72±0.37 and 0.07±0.04, respectively. The richness of fungi in naturally restored forest land was the highest, with Ace and Chao1 index of 708.19±137.25 and 706.26±125.34, respectively. The bacterial diversity and richness of species in forest land was the highest. The Shannon, Simpson, Ace and Chao1 indices were 6.57±0.04, 0.004±0.00, 3439.81±41.67, 3463.14±32.16, respectively. 3) The fungus with significant difference among restoration models were , , and . Bacteria from , , had significant difference among restoration models. 4) The RDA ordination of the dominant microbial flora and soil physical-chemical properties showed that soil bulk density (BD), carbon to nitrogen ratio (C/N), and pH were the major factors affecting the dominant fungal flora. BD, nitrogen to phosphorus ratio (N/P), total phosphorus (TP), and total carbon (TC) were the main factors affecting the dominant bacterial flora. In general, the difference of composition and diversity in the fungal community of different restoration models was higher than that of the bacterial community, indicating that the fungal communities were more sensitive to the changes of tree species and soil environment than bacterial communities. Our results could provide the theoretical foundation for vegetation restoration measures and the maintenance of ecosystem function stability in southern Ningxia.
为揭示人工林对土壤微生物环境的影响,利用高通量测序技术比较了宁夏南部山区5种恢复模式(模式1、模式2、模式3、模式4、自然恢复)下土壤真菌和细菌群落的组成及多样性。分析了土壤理化性质与优势微生物类群之间的相关性。结果表明:1)不同恢复模式下的优势真菌为子囊菌门、担子菌门、被孢霉门和未分类真菌,占真菌群落总数的90%。优势土壤细菌为放线菌门、变形菌门、酸杆菌门、绿弯菌门和其他细菌,占细菌群落总数的80%以上。2)模式3森林土壤真菌多样性最高,香农指数和辛普森指数分别为3.72±0.37和0.07±0.04。天然恢复林地的真菌丰富度最高,Ace指数和Chao1指数分别为708.19±137.25和706.26±125.34。模式4林地的细菌多样性和物种丰富度最高。香农指数、辛普森指数、Ace指数和Chao1指数分别为6.57±0.04、0.004±0.00、3439.81±41.67、3463.14±32.16。3)恢复模式间有显著差异的真菌为模式1、模式2和模式4。模式1、模式2、模式3的细菌在恢复模式间有显著差异。4)优势微生物菌群与土壤理化性质的RDA排序表明,土壤容重(BD)、碳氮比(C/N)和pH是影响优势真菌菌群的主要因素。BD、氮磷比(N/P)、总磷(TP)和总碳(TC)是影响优势细菌菌群的主要因素。总体而言,不同恢复模式下真菌群落组成和多样性的差异高于细菌群落,表明真菌群落比细菌群落对树种和土壤环境变化更敏感。研究结果可为宁夏南部植被恢复措施及生态系统功能稳定性维持提供理论依据。