从高雄海岸的海洋微藻中进行叶黄素和脂质生产的生物勘探。
Bioprospecting of marine microalgae from Kaohsiung Seacoast for lutein and lipid production.
机构信息
Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India.
Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Sustainable Environment Research Center, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Institute of Aquatic Science and Technology, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
出版信息
Bioresour Technol. 2022 May;351:126928. doi: 10.1016/j.biortech.2022.126928. Epub 2022 Mar 4.
A bioprospecting study was conducted from Seawater samples collected at Kaohsiung Seacoast, Taiwan. The current research was aimed to isolate potential lutein-producing strain, evaluate and optimize the best cultivation mode, lutein accumulation stage, lutein-extraction method, and condition to recover maximum lutein (main product) and lipid (byproduct). Biorefinery is the latest approach worldwide to extract multi-products for cost-effectiveness. Selected isolate among several isolates, identified as Chlorella sorokiniana Kh12 and exploited under biorefinery concept for lutein and lipid extraction. Kh12 cultivated under mixotrophy: 2X-(HT)-9k yielded maximum biomass (3.46 g L) and lutein (13.69 mg g) which is among the higher yields reported so far. Among various tested solvents, methanol was the best extractor. Bead milling was most effective to disrupt algal cell walls, seven minutes of milling was best for maximum lutein (7.56 mg g) extraction. Kh12 could be a promising candidate for commercial lutein and lipid co-production based on the outcome.
从台湾高雄海岸采集的海水样本中进行了一项生物勘探研究。本研究旨在分离潜在的叶黄素产生菌株，评估和优化最佳的培养模式、叶黄素积累阶段、叶黄素提取方法和条件，以最大限度地回收叶黄素（主要产物）和脂质（副产物）。生物炼制是全球最新的提取多种产品以实现成本效益的方法。从几种分离株中选择的分离株，鉴定为 Chlorella sorokiniana Kh12，并在生物炼制概念下用于叶黄素和脂质的提取。Kh12 在混合营养培养下：2X-(HT)-9k 产生最大的生物量（3.46 g L）和叶黄素（13.69 mg g），这是迄今为止报道的较高产量之一。在各种测试的溶剂中，甲醇是最好的提取剂。珠磨是破坏藻类细胞壁最有效的方法，七分钟的磨碎时间是提取最大叶黄素（7.56 mg g）的最佳时间。根据结果，Kh12 可能是一种有前途的商业叶黄素和脂质联产的候选者。
Zotero 插件