Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; Education and Research Center for Food Animal Health, Gifu University (GeFAH), 1-1 Yanagido, Gifu 501-1193, Japan.
J Microbiol Methods. 2022 Apr;195:106456. doi: 10.1016/j.mimet.2022.106456. Epub 2022 Mar 28.
Campylobacter jejuni is a globally important foodborne pathogen that can exist environmentally in a viable but non-culturable (VBNC) state, leading to missed detection of VBNC cells in food and false results in epidemiological surveillance. To establish a method for its resuscitation from the VBNC state and enable better detection, the mechanisms by which C. jejuni are induced into the VBNC state should be understood in detail. However, experimental induction of the VBNC state can be rather time consuming. Therefore, in this study, we investigated the effects of temperature, nutrition, oxygen, and osmolality, in an attempt to achieve a shorter induction time for VBNC state in C. jejuni. Culture at 4 °C under aerobic conditions in nutrient-rich Mueller-Hinton broth was the most effective condition for inducing the VBNC state in the C. jejuni strain JCM 2013. However, in C. jejuni strains 81-176 and B17, this condition induced the VBNC state slower than in the JCM 2013 strain, suggesting that the bacterial strain characteristics also affected the induction of VBNC state. These findings provide novel insights into the effects of various conditions on inducing the VBNC state in C. jejuni.
空肠弯曲菌是一种全球性重要的食源性致病菌，它可以在环境中以存活但非可培养状态（VBNC）存在，导致食品中 VBNC 细胞的漏检和流行病学监测的假阳性结果。为了从 VBNC 状态中复苏它并实现更好的检测，应该详细了解空肠弯曲菌进入 VBNC 状态的机制。然而，VBNC 状态的实验诱导可能相当耗时。因此，在这项研究中，我们研究了温度、营养、氧气和渗透压的影响，试图缩短空肠弯曲菌 VBNC 状态的诱导时间。在有氧条件下于营养丰富的 Mueller-Hinton 肉汤中于 4°C 培养是诱导空肠弯曲菌 JCM 2013 菌株 VBNC 状态的最有效条件。然而，在菌株 81-176 和 B17 中，这种条件诱导 VBNC 状态的速度比 JCM 2013 菌株慢，这表明细菌菌株特性也影响 VBNC 状态的诱导。这些发现为各种条件对空肠弯曲菌 VBNC 状态诱导的影响提供了新的见解。
