用于增强侧向流动测试条灵敏度的含纳米纤维硝酸纤维素膜改性

Modification of a nitrocellulose membrane with nanofibers for sensitivity enhancement in lateral flow test strips.

作者信息

Wang Xue, Xue Chao-Hua, Yang Dong, Jia Shun-Tian, Ding Ya-Ru, Lei Lei, Gao Ke-Yi, Jia Tong-Tong

机构信息

College of Environmental Science and Engineering, Shaanxi University of Science and Technology Xi'an 710021 China

College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology Xi'an 710021 China.

出版信息

RSC Adv. 2021 Aug 2;11(43):26493-26501. doi: 10.1039/d1ra04369b.

DOI:10.1039/d1ra04369b

PMID:35479983

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9037416/

Abstract

Lateral-flow analysis (LFA) is a convenient, low-cost, and rapid detection method, which has been widely used for screening of diseases. However, sensitivity enhancement in LFA is still a focus in this field and remains challenging. Herein, we propose an electrospinning coating method to modify the conventional nitrocellulose (NC) membrane and optimize the liquid flow rate for enhancing the sensitivity of the NC based LFA strips in the detection of human chorionic gonadotropin (HCG) and luteinizing hormone (LH). It can be seen that coating the NC membrane with nitrocellulose fibers could obtain a NC based strip with HCG and LH detection limits of 0.22 and 0.36 mIU mL respectively, and a quantitative linear range of 0.5-500 mIU mL. The results show that electrospinning is effective in modifying conventional NC membranes for LFA applications.

摘要

侧向流动分析（LFA）是一种便捷、低成本且快速的检测方法，已被广泛用于疾病筛查。然而，提高LFA的灵敏度仍是该领域的一个重点，且仍然具有挑战性。在此，我们提出一种静电纺丝涂层方法来修饰传统的硝酸纤维素（NC）膜，并优化液体流速，以提高基于NC的LFA试纸条在检测人绒毛膜促性腺激素（HCG）和促黄体生成素（LH）时的灵敏度。可以看出，用硝酸纤维素纤维涂覆NC膜可获得一种基于NC的试纸条，其对HCG和LH的检测限分别为0.22和0.36 mIU/mL，定量线性范围为0.5 - 500 mIU/mL。结果表明，静电纺丝对于修饰传统NC膜以用于LFA应用是有效的。