Baruah Rashmi Rekha, Chandra Kalita Mohan, Devi Dipali
Seri-biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology (IASST) Paschim Boragaon Guwahati 781035 India
Department of Biotechnology, Gauhati University Guwahati 781014 India.
RSC Adv. 2020 Mar 3;10(15):9070-9078. doi: 10.1039/c9ra08901b. eCollection 2020 Feb 27.
Silk fibroin (SF) is well known for its excellent biocompatible properties facilitating its application in the field of biomedical engineering through different biomaterial fabrications in the recent era. Here in this study, novel nanoparticles from non-mulberry SF of were fabricated, characterized and evaluated for its applicability as nanocarrier. Fabricated nanoparticles were initially compared with prevailing SF nanoparticles from . Fabricated silk fibroin nanoparticles (AA-SFNps) were found to be lesser in size (80-300 nm in diameter) than silk fibroin nanoparticles (BM-SFNps) (120-500 nm in diameter). When checked for stability, AA-SFNps were found to be more stable than BM-SFNps in biological media. FTIR and XRD studies revealed persistence of structural properties even after fabrication. TGA and DSC studies showed AA-SFNps to be thermally more stable than BM-SFNps without any cytotoxicity (MTT assay). On loading with model drug Doxorubicin hydrochloride (DOX), AA-SFNps exhibited an encapsulation efficiency of 94.47% with 11.81% loading of the anticancer drug. Cumulative release study revealed highest percentage release of DOX (42.1 ± 0.4%) at pH 5.2 on day 7 in comparison to pH 7.4 and 8.0. Sustained release profile of the DOX loaded AA-SFNps (AA-SFNps-DOX) was clearly reflected and it was found to be highly cytotoxic against triple negative MDA-MB-231 cells in comparison to free DOX at different time points. Overall, this study showed the efficacy of the AA-SFNps as a nanocarrier for future drug delivery applications.
丝素蛋白（SF）以其优异的生物相容性而闻名，这使得它在当代能够通过不同的生物材料制备方法应用于生物医学工程领域。在本研究中，制备了来自非桑蚕丝素蛋白的新型纳米颗粒，对其进行了表征，并评估了其作为纳米载体的适用性。最初将制备的纳米颗粒与现有的来自[具体来源未提及]的丝素蛋白纳米颗粒进行了比较。发现制备的丝素蛋白纳米颗粒（AA-SFNps）的尺寸（直径80 - 300 nm）比丝素蛋白纳米颗粒（BM-SFNps）（直径120 - 500 nm）更小。在检查稳定性时，发现AA-SFNps在生物介质中比BM-SFNps更稳定。傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）研究表明，即使在制备后结构性质仍然存在。热重分析（TGA）和差示扫描量热法（DSC）研究表明，AA-SFNps在热稳定性上比BM-SFNps更高，且没有任何细胞毒性（MTT法测定）。用模型药物盐酸多柔比星（DOX）负载后，AA-SFNps表现出94.47%的包封率，抗癌药物的负载量为11.81%。累积释放研究表明，与pH 7.4和8.0相比，在第7天pH 5.2时DOX的释放百分比最高（42.1 ± 0.4%）。负载DOX的AA-SFNps（AA-SFNps-DOX）的缓释曲线清晰可见，并且发现在不同时间点与游离DOX相比，它对三阴性MDA-MB-231细胞具有高度细胞毒性。总体而言，本研究表明AA-SFNps作为未来药物递送应用的纳米载体具有有效性。
