伊朗东北部地区[具体菌种名称缺失]和[具体菌种名称缺失]分离株中唑类耐药的高发生率。
High incidence of azole resistance among and isolates in Northeastern Iran.
作者信息
Zarrinfar Hossein, Kord Zahra, Fata Abdolmajid
机构信息
Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Curr Med Mycol. 2021 Sep;7(3):18-21. doi: 10.18502/cmm.7.3.7801.
BACKGROUND AND PURPOSE
Resistance to antifungal drugs is increasing among isolates from patients with vulvovaginal candidiasis (VVC). Lack of correct diagnosis of causing VVC and the experimental use of antifungal drugs are the main causes of this resistance. This study aimed to determine the susceptibility of antifungal drugs against species isolated from VVC in Northeastern Iran.
MATERIALS AND METHODS
Among women suspected of VVC, 189 vaginal discharge specimens were evaluated. isolates detected by polymerase chain reaction-restriction fragment length polymorphism were examined by standard antifungal disk diffusion susceptibility testing method for voriconazole, itraconazole, fluconazole, and ketoconazole. The susceptibility pattern of these antifungals was reported as sensitive, susceptible dose-dependent, and resistant. The results were evaluated by SPSS software and analyzed by Pearson chi-squared test.
RESULTS
Among the vaginal specimens, 108 out of 189 isolates were identified as , , , , and . The susceptibility rates of isolates to voriconazole, ketoconazole, itraconazole, and fluconazole were 92.6%, 90.7%, 68.5%, and 63.9%, respectively. Moreover, the resistance rates to fluconazole, itraconazole, ketoconazole, and itraconazole were 15.7%, 8.3%, 1.9%, and 1.9%, respectively. The and isolates were resistant to antifungal discs among 93% and 20% of the specimens, respectively.
CONCLUSION
The and species showed the highest resistance to antifungal drugs. Furthermore, isolates showed the highest sensitivity to voriconazole and ketoconazole and the lowest sensitivity to fluconazole.
背景与目的
外阴阴道念珠菌病（VVC）患者分离出的菌株对抗真菌药物的耐药性正在增加。导致VVC的病因诊断不正确以及抗真菌药物的经验性使用是这种耐药性的主要原因。本研究旨在确定抗真菌药物对从伊朗东北部VVC患者中分离出的菌种的敏感性。
材料与方法
在疑似VVC的女性中，对189份阴道分泌物标本进行了评估。通过聚合酶链反应-限制性片段长度多态性检测到的分离株，采用标准抗真菌纸片扩散药敏试验方法检测伏立康唑、伊曲康唑、氟康唑和酮康唑。这些抗真菌药物的药敏模式报告为敏感、剂量依赖性敏感和耐药。结果通过SPSS软件进行评估，并采用Pearson卡方检验进行分析。
结果
在阴道标本中，189株分离株中有108株被鉴定为白色念珠菌、热带念珠菌、光滑念珠菌、近平滑念珠菌和季也蒙念珠菌。分离株对伏立康唑、酮康唑、伊曲康唑和氟康唑的敏感率分别为92.6%、90.7%、68.5%和63.9%。此外，对氟康唑、伊曲康唑、酮康唑和伊曲康唑的耐药率分别为15.7%、8.3%、1.9%和1.9%。光滑念珠菌和克柔念珠菌分离株分别在93%和20%的标本中对抗真菌纸片耐药。
结论
光滑念珠菌和克柔念珠菌对抗真菌药物的耐药性最高。此外，分离株对伏立康唑和酮康唑的敏感性最高，对氟康唑的敏感性最低。
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