Husin Naimah, Sapak Zaiton
Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Jasin Campus, 77300 Merlimau, Melaka, Malaysia.
Sustainable Crop Protection Research Group, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
Trop Life Sci Res. 2022 Mar;33(1):77-89. doi: 10.21315/tlsr2022.33.1.5. Epub 2022 Mar 31.
Bacterial heart rot (BHR) disease caused by pathogenic bacteria, , is one of the destructive diseases of pineapple worldwide. This study explored the potential of against the BHR pathogen and . The BHR causal pathogen was isolated from symptomatic pineapple plants, demonstrating water-soaked and rotten basal tissues. Biological control agent (BCA) was isolated from asymptomatic pineapple leaves, later confirmed as , and subsequently tested for the antagonistic activity against the BHR pathogen via disc diffusion assay and glasshouse trial. showed the ability to inhibit the growth of BHR pathogen with 18.10 ± 0.36 mm of inhibition zone in diameter. The ability of against the BHR pathogen was further confirmed via the glasshouse trial with five treatments. The results showed that treatments with inoculation recorded lower disease severity index of 0.04 ± 0.01 than the positive control treatment with pathogen alone (0.53 ± 0.04). This finding indicated that has a great potential as BCA against BHR disease in pineapple var. MD2, however, the effectiveness of this isolate needs to be further tested under actual field conditions.
由致病细菌引起的细菌性心腐病（BHR）是全球菠萝的毁灭性病害之一。本研究探索了[具体生物防治剂名称未给出]对BHR病原菌[具体病原菌名称未给出]和[具体病原菌名称未给出]的防治潜力。从有症状的菠萝植株中分离出BHR致病病原菌，这些植株表现出基部组织水渍状和腐烂。从无症状的菠萝叶片中分离出生物防治剂（BCA），后来鉴定为[具体生物防治剂名称未给出]，随后通过纸片扩散法和温室试验测试其对BHR病原菌的拮抗活性。[具体生物防治剂名称未给出]显示出抑制BHR病原菌生长的能力，抑菌圈直径为18.10±0.36毫米。通过有五种处理的温室试验进一步证实了[具体生物防治剂名称未给出]对BHR病原菌的防治能力。结果表明，接种[具体生物防治剂名称未给出]的处理病害严重程度指数较低，为0.04±0.01，低于仅接种病原菌的阳性对照处理（0.53±0.04）。这一发现表明，[具体生物防治剂名称未给出]作为防治菠萝品种MD2细菌性心腐病的生物防治剂具有很大潜力，然而，该分离株的有效性需要在实际田间条件下进一步测试。
