State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Biosensors (Basel). 2022 Jun 15;12(6):419. doi: 10.3390/bios12060419.
Hydroxyapatite (HAP) materials are widely applied as biomedical materials due to their stable performance, low cost, good biocompatibility and biodegradability. Here, a green, fast and efficient strategy was designed to construct a fluorescent nanosystem for cell imaging and drug delivery based on polyethyleneimine (PEI) and functionalized HAP via simple physical adsorption. First, HAP nanorods were functionalized with riboflavin sodium phosphate (HE) to provide them with fluorescence properties based on ligand-exchange process. Next, PEI was attached on the surface of HE-functionalized HAP (HAP-HE@PEI) via electrostatic attraction. The fluorescent HAP-HE@PEI nanosystem could be rapidly taken up by NIH-3T3 fibroblast cells and successfully applied to for cell imaging. Additionally, doxorubicin hydrochloride (DOX) containing HAP-HE@PEI with high loading capacity was prepared, and in-vitro release results show that the maximum release of DOX at pH 5.4 (31.83%) was significantly higher than that at pH 7.2 (9.90%), which can be used as a drug delivery tool for cancer therapy. Finally, HAP-HE@PEI as the 3D inkjet printing ink were printed with GelMA hydrogel, showing a great biocompatible property for 3D cell culture of RAW 264.7 macrophage cells. Altogether, because of the enhanced affinity with the cell membrane of HAP-HE@PEI, this green, fast and efficient strategy may provide a prospective candidate for bio-imaging, drug delivery and bio-printing.
羟基磷灰石（HAP）材料由于其性能稳定、成本低、生物相容性和可生物降解性好而被广泛应用于生物医学领域。在这里，设计了一种绿色、快速、高效的策略，通过简单的物理吸附作用，基于聚乙烯亚胺（PEI）和功能化 HAP 构建用于细胞成像和药物输送的荧光纳米系统。首先，通过配体交换过程，使 HAP 纳米棒与核黄素磷酸钠（HE）功能化，从而赋予其荧光性能。接下来，通过静电吸引将 PEI 附着在 HE 功能化 HAP（HAP-HE@PEI）的表面上。荧光 HAP-HE@PEI 纳米系统可以被 NIH-3T3 成纤维细胞快速摄取，并成功应用于细胞成像。此外，制备了载有高负载量盐酸阿霉素（DOX）的 HAP-HE@PEI，体外释放结果表明，在 pH 5.4（31.83%）时 DOX 的最大释放量明显高于在 pH 7.2（9.90%）时，可作为癌症治疗的药物输送工具。最后，将 HAP-HE@PEI 用作 3D 喷墨打印墨水与 GelMA 水凝胶一起打印，显示出对 RAW 264.7 巨噬细胞的 3D 细胞培养的良好生物相容性。总之，由于 HAP-HE@PEI 与细胞膜的增强亲和力，这种绿色、快速、高效的策略可能为生物成像、药物输送和生物打印提供有前景的候选物。
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