肿瘤治疗电场与氧化锌纳米粒子对三种不同人癌细胞系细胞凋亡和遗传毒性的协同作用。

Synergetic Effect of Tumor Treating Fields and Zinc Oxide Nanoparticles on Cell Apoptosis and Genotoxicity of Three Different Human Cancer Cell Lines.

机构信息

Medical Biophysics Department, Medical Research Institute, Alexandria University, Alexandria 21561, Egypt.

Alexandria University Cancer Research Cluster, Alexandria 21561, Egypt.

出版信息

Molecules. 2022 Jul 8;27(14):4384. doi: 10.3390/molecules27144384.

DOI:10.3390/molecules27144384

PMID:35889257

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9322763/

Abstract

Cancer remains a leading cause of death worldwide, despite extraordinary progress. So, new cancer treatment modalities are needed. Tumor-treating fields (TTFs) use low-intensity, intermediate-frequency alternating electric fields with reported cancer anti-mitotic properties. Moreover, nanomedicine is a promising therapy option for cancer. Numerous cancer types have been treated with nanoparticles, but zinc oxide nanoparticles (ZnO NPs) exhibit biocompatibility. Here, we investigate the activity of TTFs, a sub-lethal dose of ZnO NPs, and their combination on hepatocellular carcinoma (HepG2), the colorectal cancer cell line (HT-29), and breast cancer cell lines (MCF-7). The lethal effect of different ZnO NPs concentrations was assessed by sulforhodamine B sodium salt assay (SRB). The cell death percent was determined by flow cytometer, the genotoxicity was evaluated by comet assay, and the total antioxidant capacity was chemically measured. Our results show that TTFs alone cause cell death of 14, 8, and 17% of HepG2, HT-29, and MCF-7, respectively; 10 µg/mL ZnO NPs was the sub-lethal dose according to SRB results. The combination between TTFs and sub-lethal ZnO NPs increased the cell death to 29, 20, and 33% for HepG2, HT-29, and MCF-7, respectively, without reactive oxygen species increase. Increasing NPs potency using TTFs can be a novel technique in many biomedical applications.

摘要

尽管取得了非凡的进展，但癌症仍然是全球主要的死亡原因。因此，需要新的癌症治疗方法。肿瘤治疗电场（TTFs）使用低强度、中频交替电场，具有报道的抗癌有丝分裂特性。此外，纳米医学是癌症治疗的一种很有前途的选择。许多癌症类型都已用纳米颗粒治疗，但氧化锌纳米颗粒（ZnO NPs）具有生物相容性。在这里，我们研究了 TTFs、亚致死剂量的 ZnO NPs 及其组合对肝癌（HepG2）、结直肠癌细胞系（HT-29）和乳腺癌细胞系（MCF-7）的活性。不同 ZnO NPs 浓度的致死效应通过磺基罗丹明 B 钠盐测定法（SRB）进行评估。通过流式细胞仪确定细胞死亡百分比，通过彗星试验评估遗传毒性，并用化学方法测量总抗氧化能力。我们的结果表明，TTFs 单独作用导致 HepG2、HT-29 和 MCF-7 的细胞死亡率分别为 14%、8%和 17%；根据 SRB 结果，10 µg/mL ZnO NPs 是亚致死剂量。TTFs 与亚致死剂量 ZnO NPs 的组合使 HepG2、HT-29 和 MCF-7 的细胞死亡率分别增加到 29%、20%和 33%，而没有增加活性氧物种。使用 TTFs 增加 NPs 的效力可能是许多生物医学应用中的一种新方法。