Hulin Michelle T, Vadillo Dieguez Andrea, Cossu Francesca, Lynn Samantha, Russell Karen, Neale Helen C, Jackson Robert W, Arnold Dawn L, Mansfield John W, Harrison Richard J
NIAB EMR East Malling UK.
Present address: The Sainsbury Laboratory Norwich UK.
Plant Pathol. 2022 May;71(4):949-965. doi: 10.1111/ppa.13513. Epub 2021 Dec 21.
Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry () production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance in sweet cherry is discernible using shoot or whole tree inoculations; however, these quantitative differences in resistance are not evident in detached leaf assays. To identify novel sources of resistance to canker, we used a rapid leaf pathogenicity test to screen a range of wild cherry, ornamental species and sweet cherry × ornamental cherry hybrids with the canker pathogens, pvs , races 1 and 2, and . Several accessions exhibited limited symptom development following inoculation with each of the pathogens, and this resistance extended to 16 . strains pathogenic on sweet cherry and plum. Resistance was associated with reduced bacterial multiplication after inoculation, a phenotype similar to that of commercial sweet cherry towards nonhost strains of . . Progeny resulting from a cross of a resistant ornamental species with susceptible sweet cherry (. ) exhibited resistance indicating it is an inherited trait. Identification of accessions with resistance to the major bacterial canker pathogens is the first step towards characterizing the underlying genetic mechanisms of resistance and introducing these traits into commercial germplasm.
细菌性溃疡病是核果类的一种主要病害，是全球甜樱桃（）生产的关键限制因素。病害防治的一项重要策略是培育抗病品种。通过嫩枝或整株接种可看出甜樱桃存在部分品种抗性；然而，在离体叶片试验中，这些抗性的数量差异并不明显。为了鉴定新的溃疡病抗性来源，我们使用快速叶片致病性试验，用溃疡病菌、致病型1和2以及对一系列野生樱桃、观赏物种和甜樱桃×观赏樱桃杂种进行筛选。接种每种病原菌后，有几个种质表现出有限的症状发展，这种抗性扩展到对甜樱桃和李致病的16个菌株。抗性与接种后细菌繁殖减少有关，这一表型与商业甜樱桃对非寄主菌株的表型相似。抗性观赏物种与感病甜樱桃（. ）杂交产生的后代表现出抗性，表明这是一种可遗传的性状。鉴定对主要细菌性溃疡病病原菌具有抗性的种质是表征抗性潜在遗传机制并将这些性状引入商业种质的第一步。
