070103 发酵乳通过调节小鼠肠道微生物群改善葡萄糖和脂代谢紊乱。

070103 Fermented Milk Improve Glucose and Lipid Metabolism Disorders by Regulating Gut Microbiota in Mice.

机构信息

College of Food Science, South China Agricultural University, Guangzhou 510642, China.

Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.

出版信息

Nutrients. 2022 Sep 29;14(19):4050. doi: 10.3390/nu14194050.

DOI:10.3390/nu14194050

PMID:36235706

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9573661/

Abstract

BACKGROUND

Fermented milk is beneficial for metabolic disorders, while the underlying mechanisms of action remain unclear. This study explored the benefits and underlying mechanisms of 070103 fermented milk (BLFM) in thirteen-week high-fat and high-sugar (HFHS) fed mice using omics techniques.

METHODS AND RESULTS

BLFM with activated glucokinase (GK) was screened by a double-enzyme coupling method. After supplementing BLFM with 10 mL/kg BW per day, fasting blood glucose, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and leptin were significantly reduced compared with the HFHS group. Among them, the final body weight (BW), epididymal fat, perirenal fat, and brown fat in BLFM group had better change trends than GG fermented milk (LGGFM) group. The amplicon and metabolomic data analysis identified as a key gut microbiota at regulating glycolipid metabolism. BLFM reverses HFHS-induced reduction in bifidobacteria abundance. Further studies showed that BLFM significantly reduces the content of 3-indoxyl sulofphate associated with intestinal barrier damage. In addition, mice treated with BLFM improved BW, glucose tolerance, insulin resistance, and hepatic steatosis.

CONCLUSION

BLFM consumption attenuates obesity and related symptoms in HFHS-fed mice probably via the modulation of gut microbes and metabolites.

摘要

背景

发酵乳有益于代谢紊乱，但其作用机制尚不清楚。本研究采用组学技术探讨了 070103 发酵乳（BLFM）在十三周高脂高糖（HFHS）喂养小鼠中的益处及其潜在机制。

方法和结果

采用双酶偶联法筛选具有活性葡萄糖激酶（GK）的 BLFM。每天补充 BLFM10mL/kg BW 后，与 HFHS 组相比，空腹血糖、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和瘦素显著降低。其中，BLFM 组的最终体重（BW）、附睾脂肪、肾周脂肪和棕色脂肪的变化趋势优于 GG 发酵乳（LGGFM）组。扩增子和代谢组学数据分析表明是调节糖脂代谢的关键肠道微生物群。BLFM 逆转了 HFHS 诱导的双歧杆菌丰度降低。进一步的研究表明，BLFM 显著降低了与肠道屏障损伤相关的 3-吲哚硫酸酯的含量。此外，BLFM 处理的小鼠改善了 BW、葡萄糖耐量、胰岛素抵抗和肝脂肪变性。