克罗地亚城市地区塔希纳正布尼亚病毒监测——“同一健康”方法

Surveillance of Tahyna Orthobunyavirus in Urban Areas in Croatia-The "One Health" Approach.

作者信息

Stevanovic Vladimir, Vilibic-Cavlek Tatjana, Savic Vladimir, Klobucar Ana, Kovac Snjezana, Curman Posavec Marcela, Petrinic Suncica, Bogdanic Maja, Santini Marija, Tesic Vanja, de Albuquerque Soares Nathalia, Barbic Ljubo

机构信息

Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.

Department of Virology, Croatian Institute of Public Health, 10000 Zagreb, Croatia.

出版信息

Trop Med Infect Dis. 2022 Oct 20;7(10):320. doi: 10.3390/tropicalmed7100320.

DOI:10.3390/tropicalmed7100320

PMID:36288061

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9609863/

Abstract

BACKGROUND

Tahyna orthobunyavirus (TAHV) is a neglected mosquito-borne bunyavirus. Although the virus is widespread in continental Europe, TAHV infections are rarely reported. We analyzed the prevalence of TAHV in humans and different animal species as well as mosquitoes collected in urban areas of Zagreb and its surroundings in the period from 2020 to 2022.

METHODS

The study included 32 patients with neuroinvasive disease (NID), 218 asymptomatic individuals, 98 horses, 94 pet animals (dogs and cats), and 4456 mosquitoes. Cerebrospinal fluid (CSF) and urine samples of patients with NID were tested for the TAHV RNA using a real-time reverse transcription-polymerase chain reaction (RT-qPCR). Human and animal serum samples were tested for TAHV-neutralizing (NT) antibodies using a virus-neutralization test (VNT). Mosquito pools were tested for TAHV RNA using an RT-qPCR.

RESULTS

TAHV NT antibodies were detected in 3/9.4% of patients with NID, 8/3.7% of asymptomatic individuals, 29/29.6% of horses, and 11/11.7% of pet animals. There was no difference in the seroprevalence according to age, sex, and area of residence in asymptomatic individuals. In addition, TAHV seropositivity did not differ according to age and sex in pet animals. None of the tested mosquito pools was TAHV RNA-positive.

CONCLUSIONS

The presented results highlight the importance of interdisciplinary surveillance ("One Health") of this neglected viral zoonosis.

摘要

背景

塔希纳正布尼亚病毒（TAHV）是一种被忽视的蚊媒传播布尼亚病毒。尽管该病毒在欧洲大陆广泛传播，但TAHV感染的报告却很少。我们分析了2020年至2022年期间在萨格勒布及其周边城市地区采集的人类、不同动物物种以及蚊子中TAHV的流行情况。

方法

该研究纳入了32例神经侵袭性疾病（NID）患者、218例无症状个体、98匹马、94只宠物动物（狗和猫）以及4456只蚊子。使用实时逆转录-聚合酶链反应（RT-qPCR）检测NID患者的脑脊液（CSF）和尿液样本中的TAHV RNA。使用病毒中和试验（VNT）检测人类和动物血清样本中的TAHV中和（NT）抗体。使用RT-qPCR检测蚊群中的TAHV RNA。