Key Laboratory of Grassland Ecosystem of Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou, 730070, China.
BMC Plant Biol. 2022 Nov 2;22(1):509. doi: 10.1186/s12870-022-03883-4.
Poa pratensis is one of the most common cold-season turfgrasses used for urban turf building, and it is also widely used in ecological environment management worldwide. Powdery mildew is a common disease of P. pratensis. To scientifically and ecologically control lawn powdery mildew, the molecular mechanism underlying the response of P. pratensis to powdery mildew infection must better understood.
To explore molecular mechanism underlying the response of P. pratensis to powdery mildew infection, this study compared physiological changes and transcriptomic level differences between the highly resistant variety 'BlackJack' and the extremely susceptible variety 'EverGlade' under powdery mildew infection conditions. We analyzed DEGs using reference canonical pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and the results showed that "starch and sucrose metabolism", "photosynthesis" and "fatty acid metabolism"pathways were only enriched in 'BlackJack', and the expression of DEGs such as HXK, INV, GS, SS, AGpase and β-amylase in "starch and sucrose metabolism" pathway of 'BlackJack' were closely related to powdery mildew resistance. Meanwhile, compared with 'EverGlade', powdery mildew infection promoted synthesis of sucrose, expression of photosynthesis parameters and photosynthesis-related enzymes in leaves of 'BlackJack' and decreased accumulation of monosaccharides such as glucose and fructose.
This study identified the key metabolic pathways of a P. pratensis variety with high resistance to powdery mildew infection and explored the differences in physiological characteristics and key genes related to sugar metabolism pathways under powdery mildew stress. These findings provide important insights for studying underlying molecular response mechanism.
草地早熟禾是用于城市草坪建设的最常见冷季草坪草之一，在全球范围内也广泛用于生态环境管理。白粉病是草地早熟禾的一种常见病。为了科学和生态地控制草坪白粉病，必须更好地了解草地早熟禾对白粉病感染的反应的分子机制。
为了探讨草地早熟禾对白粉病感染的反应的分子机制，本研究比较了高抗品种“BlackJack”和极感品种“EverGlade”在白粉病感染条件下的生理变化和转录组水平差异。我们使用京都基因与基因组百科全书（KEGG）数据库中的参考规范途径分析了 DEGs，结果表明，“淀粉和蔗糖代谢”、“光合作用”和“脂肪酸代谢”途径仅在“BlackJack”中富集，并且“淀粉和蔗糖代谢”途径中 HXK、INV、GS、SS、AGpase 和β-淀粉酶等 DEGs 的表达与白粉病抗性密切相关。同时，与“EverGlade”相比，白粉病感染促进了蔗糖的合成，“BlackJack”叶片中光合作用参数和相关酶的表达，以及葡萄糖和果糖等单糖的积累减少。
本研究鉴定了对白粉病感染具有高抗性的草地早熟禾品种的关键代谢途径，并探讨了白粉病胁迫下与糖代谢途径相关的生理特征和关键基因的差异。这些发现为研究潜在的分子反应机制提供了重要的见解。
