Department of Surgery, Kyushu University Beppu Hospital, Beppu 874-0838, Japan; Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita 565-0871, Japan.
Division of Systems Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Cell Rep. 2023 Jan 31;42(1):111929. doi: 10.1016/j.celrep.2022.111929. Epub 2023 Jan 18.
The cellular interactions in the tumor microenvironment of colorectal cancer (CRC) are poorly understood, hindering patient treatment. In the current study, we investigate whether events occurring at the invasion front are of particular importance for CRC treatment strategies. To this end, we analyze CRC tissues by combining spatial transcriptomics from patients with a public single-cell transcriptomic atlas to determine cell-cell interactions at the invasion front. We show that CRC cells are localized specifically at the invasion front. These cells induce human leukocyte antigen G (HLA-G) to produce secreted phosphoprotein 1 (SPP1)+ macrophages while conferring CRC cells with anti-tumor immunity, as well as proliferative and invasive properties. Taken together, these findings highlight the signaling between CRC cell populations and stromal cell populations at the cellular level.
结直肠癌（CRC）肿瘤微环境中的细胞相互作用知之甚少，这阻碍了患者的治疗。在本研究中，我们研究了侵袭前沿发生的事件是否对 CRC 治疗策略特别重要。为此，我们通过将来自患者的空间转录组学与公共单细胞转录组图谱相结合来分析 CRC 组织，以确定侵袭前沿的细胞-细胞相互作用。我们表明，CRC 细胞特异性定位于侵袭前沿。这些细胞诱导人类白细胞抗原 G（HLA-G）产生分泌型磷蛋白 1（SPP1）+巨噬细胞，同时赋予 CRC 细胞抗肿瘤免疫以及增殖和侵袭特性。总之，这些发现强调了 CRC 细胞群体和基质细胞群体在细胞水平上的信号传递。
