Dietrich Bianca, Kunihs Victoria, Pollheimer Jürgen, Knöfler Martin, Haider Sandra
Department of Obstetrics and Gynaecology, Reproductive Biology Unit, Placental Development Group, Medical University of Vienna, Vienna, Austria.
Department of Obstetrics and Gynaecology, Reproductive Biology Unit, Maternal-Fetal Immunology Group, Medical University of Vienna, Vienna, Austria.
Placenta. 2023 Mar 3;133:19-22. doi: 10.1016/j.placenta.2023.01.005. Epub 2023 Jan 13.
3-dimensional trophoblast organoids (TB-ORG) represent a reliable model for studying extravillous trophoblast (EVT) lineage formation and differentiation. However, restricted access to first trimester placentae requires alternative cell sources for establishing placental organoids. Recently, we demonstrated EVT differentiation in JEG-3-derived organoids. Consequently, we herein tested whether other commonly used trophoblastic cell lines, ACH-3P, HTR-8/SVneo, and SWAN-71 were capable of self-organizing into organoids and subsequent EVT differentiation. Notably, only ACH-3P formed organoids under stemness conditions mimicking TB-ORG architectures, and induction of EVT differentiation provoked formation of HLA-G areas. Hence ACH-3P-ORGs provide another organoid model for studying controlled EVT lineage formation and differentiation.
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