Dietrich Bianca, Kunihs Victoria, Pollheimer Jürgen, Knöfler Martin, Haider Sandra
Department of Obstetrics and Gynaecology, Reproductive Biology Unit, Placental Development Group, Medical University of Vienna, Vienna, Austria.
Department of Obstetrics and Gynaecology, Reproductive Biology Unit, Maternal-Fetal Immunology Group, Medical University of Vienna, Vienna, Austria.
Placenta. 2023 Mar 3;133:19-22. doi: 10.1016/j.placenta.2023.01.005. Epub 2023 Jan 13.
3-dimensional trophoblast organoids (TB-ORG) represent a reliable model for studying extravillous trophoblast (EVT) lineage formation and differentiation. However, restricted access to first trimester placentae requires alternative cell sources for establishing placental organoids. Recently, we demonstrated EVT differentiation in JEG-3-derived organoids. Consequently, we herein tested whether other commonly used trophoblastic cell lines, ACH-3P, HTR-8/SVneo, and SWAN-71 were capable of self-organizing into organoids and subsequent EVT differentiation. Notably, only ACH-3P formed organoids under stemness conditions mimicking TB-ORG architectures, and induction of EVT differentiation provoked formation of HLA-G areas. Hence ACH-3P-ORGs provide another organoid model for studying controlled EVT lineage formation and differentiation.
三维滋养层类器官（TB-ORG）是研究绒毛外滋养层（EVT）谱系形成和分化的可靠模型。然而，由于获取孕早期胎盘的途径有限，需要替代细胞来源来建立胎盘类器官。最近，我们证明了JEG-3衍生的类器官中存在EVT分化。因此，我们在此测试了其他常用的滋养层细胞系ACH-3P、HTR-8/SVneo和SWAN-71是否能够自我组织形成类器官并随后进行EVT分化。值得注意的是，只有ACH-3P在模拟TB-ORG结构的干性条件下形成了类器官，并且EVT分化的诱导引发了HLA-G区域的形成。因此，ACH-3P-ORGs为研究可控的EVT谱系形成和分化提供了另一种类器官模型。
