Wilmes F, Hossmann K A
Acta Neuropathol. 1979 Jan 12;45(1):47-51. doi: 10.1007/BF00691804.
Serum proteins as constituents of vasogenic brain edema were visualized both macroscopically and microscopically applying a double layer immunofluorescence technique to paraffin embedded material derived from three experimental series: peritumorous edema following xeno-transplantation of glioma cells, edema after cerebral embolization with micropheres, and edema after unilateral MCA occlusion. Exclusively cats were used as experimental animals. The staining procedures resulted in selective green fluorescence of vessel contents as well as edema protein, which was demonstrable even macroscopically at times, where edema formation reaches a maximum in each experimental series. Microscopically, serum protein could be traced up to the end of observation time ranging from 1 to 4 weeks, where the specific fluorescence was related to cellular structures. As compared to other techniques employed in brain edema localization, immunostaining mainly offers the following advantages: avoidance of in vivo tracing, better structural resolution in paraffin than in freeze sections, high specificity and sensitivity in antigen localization.
应用双层免疫荧光技术对石蜡包埋材料进行处理，该材料取自三个实验系列：胶质瘤细胞异种移植后的瘤周水肿、微球脑栓塞后的水肿以及大脑中动脉单侧闭塞后的水肿，从而在宏观和微观层面观察血清蛋白作为血管源性脑水肿的组成成分。实验动物仅使用猫。染色程序使得血管内容物以及水肿蛋白产生选择性绿色荧光，在每个实验系列中水肿形成达到最大值时，有时甚至在宏观层面也可显示出来。在显微镜下，血清蛋白在长达1至4周的观察期内均可追踪到，此时特异性荧光与细胞结构相关。与用于脑水肿定位的其他技术相比，免疫染色主要具有以下优点：避免体内追踪、石蜡切片比冷冻切片具有更好的结构分辨率、抗原定位具有高特异性和高灵敏度。
