Duckett Drew J, Sullivan Jack, Pirro Stacy, Carstens Bryan C
Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, 1315 Kinnear Rd., Columbus, OH 43212, USA.
Department of Biological Sciences, University of Idaho, Box 443051, Moscow, ID 83844-3051, USA.
GigaByte. 2021 May 6;2021:gigabyte19. doi: 10.46471/gigabyte.19. eCollection 2021.
Voles of the genus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole () and from the montane vole (). The water vole genomes were sequenced with Illumina and 10× Chromium plus Illumina sequencing, resulting in assemblies with ∼1600,000 and ∼30,000 scaffolds, respectively. The montane vole was also assembled into ∼13,000 scaffolds using Illumina sequencing. Mitochondrial genome assemblies were also performed for both species. Structural and functional annotation for the best water vole nuclear genome resulted in ∼24,500 annotated genes, with 83% of these having functional annotations. Assembly quality statistics for our nuclear assemblies fall within the range of genomes previously published in the genus , making the water vole and montane vole genomes useful additions to currently available genomic resources.
水田鼠属的田鼠是重要的研究生物，但缺乏基因组资源。这些资源将有益于未来的免疫学、系统地理学、隐秘多样性等方面的研究。我们对水田鼠（）的两个亚种和山地田鼠（）的核基因组进行了测序和组装。水田鼠基因组采用Illumina和10×Chromium加Illumina测序，分别得到了约160万个和约3万个支架的组装结果。山地田鼠也使用Illumina测序组装成了约1.3万个支架。还对这两个物种进行了线粒体基因组组装。对最佳水田鼠核基因组的结构和功能注释产生了约24500个注释基因，其中83%具有功能注释。我们核组装的组装质量统计数据落在该属先前发表的基因组范围内，这使得水田鼠和山地田鼠基因组成为现有基因组资源的有益补充。
