Centre for Clinical Research, North Denmark Regional Hospital, Hjoerring, Denmark.
Department of Clinical Medicine, Aalborg University, Hjoerring, Denmark.
Gut Microbes. 2023 Jan-Dec;15(1):2211923. doi: 10.1080/19490976.2023.2211923.
An association has been suggested between altered gut microbiota, and attention deficit hyperactivity disorder (ADHD), and autism spectrum disorder (ASD), respectively. Thus, we analyzed the gut microbiota composition in children and adolescents with or without these disorders and evaluated the systemic effects of these bacteria. We recruited study participants diagnosed with ADHD, ASD, and comorbid ADHD/ASD, while the control groups consisted both of siblings and non-related children. The gut microbiota was analyzed by 16S rRNA gene sequencing of the V4 region, while the concentration of lipopolysaccharide-binding protein (LBP), cytokines, and other signaling molecules were measured in plasma. Importantly the gut microbiota compositions of cases with ADHD and ASD were highly similar for both alpha- and beta-diversity while differing from that of non-related controls. Furthermore, a subset of ADHD and ASD cases had an increased LBP concentration compared to non-affected children, which was positively correlated with interleukin (IL)-8, 12, and 13. These observations indicate disruption of the intestinal barrier and immune dysregulation among the subset of children with ADHD or ASD.
有人提出，肠道微生物群的改变分别与注意力缺陷多动障碍（ADHD）和自闭症谱系障碍（ASD）有关。因此，我们分析了患有或不患有这些疾病的儿童和青少年的肠道微生物群组成，并评估了这些细菌的全身效应。我们招募了被诊断患有 ADHD、ASD 和共患 ADHD/ASD 的研究参与者，而对照组则包括兄弟姐妹和非相关儿童。通过对 V4 区 16S rRNA 基因进行测序来分析肠道微生物群，同时测量血浆中脂多糖结合蛋白 (LBP)、细胞因子和其他信号分子的浓度。重要的是，ADHD 和 ASD 病例的肠道微生物群组成在 alpha 和 beta 多样性方面高度相似，而与非相关对照组不同。此外，一部分 ADHD 和 ASD 病例的 LBP 浓度与未受影响的儿童相比有所增加，且与白细胞介素 (IL)-8、12 和 13 呈正相关。这些观察结果表明，在 ADHD 或 ASD 儿童的亚组中存在肠道屏障破坏和免疫失调。
