PKDERM Laboratories, 06130 Grasse, France.
Probiotic Group Luxembourg S.A., Research and Development Department, 9944 Weiswampach, Luxembourg.
Int J Mol Sci. 2023 Aug 2;24(15):12360. doi: 10.3390/ijms241512360.
The use of probiotics has gained increasing attention as a strategy for wound healing to decrease microbial resistance to disinfectants and antibiotics. This study aimed to investigate the potential of a non-medicinal topical cocktail of probiotic bacteria (CPB) in promoting wound healing in dogs using in vitro scratch assay. Canine Progenitors Epidermal Keratinocytes (CPEK) were exposed to a prototype product formulated with CPB (PPP), non-formulated CPB, and the vehicle. The viability of CPB and CPEK cells was first evaluated in the co-culture model. Then, wound closure was analyzed over time. The CPB required a minimum concentration of 75 CFU/mL for better viability with CPEK. While the CPEK preserved 100% of their viability when PPP was diluted to up to 75,000 CFU/mL. At higher concentrations, the viability of CPEK was reduced by the concomitant effect of the non-formulated CPB and the vehicle. The formulated and non-formulated CPB and the vehicle seem to lead to a dose-dependent increase in cell migration compared to the control. Importantly, at the concentration of 750,000 CFU/mL, the PPP showed a 20% increase in wound closure. Taken together, our findings suggest the potential beneficial effects of the probiotic-based topical cocktail (PPP) on wound healing. However, to confirm and validate these effects, further experiments are necessary to provide more robust evidence and allow us to confidently establish the potential beneficial effects of the probiotic bacteria (CPB) in promoting wound healing.
使用益生菌作为一种减少微生物对抗生素和消毒剂耐药性的策略，以促进伤口愈合，已引起越来越多的关注。本研究旨在通过体外划痕试验，研究非药用局部益生菌混合物（CPB）在促进犬伤口愈合中的潜力。将犬原代表皮角质形成细胞（CPEK）暴露于含有 CPB（PPP）、未配制 CPB 和载体的原型产品中。首先在共培养模型中评估 CPB 和 CPEK 细胞的活力。然后，随着时间的推移分析伤口闭合。CPB 与 CPEK 共培养时，需要最低浓度为 75 CFU/mL，才能获得更好的活力。当 PPP 稀释至高达 75,000 CFU/mL 时，CPEK 保留了 100%的活力。在更高的浓度下，CPEK 的活力由于未配制 CPB 和载体的协同作用而降低。与对照组相比，配制和未配制的 CPB 和载体似乎导致细胞迁移呈剂量依赖性增加。重要的是，在 750,000 CFU/mL 的浓度下，PPP 显示出 20%的伤口闭合增加。综上所述，我们的研究结果表明，基于益生菌的局部混合物（PPP）对伤口愈合具有潜在的有益作用。然而，为了证实和验证这些作用，需要进一步的实验提供更有力的证据，并使我们能够有信心确定益生菌（CPB）在促进伤口愈合中的潜在有益作用。
