电针对心肌缺血模型大鼠心功能的改善作用：下丘脑-垂体-肾上腺轴的潜在作用。
Electroacupuncture ameliorates cardiac dysfunction in myocardial ischemia model rats: a potential role of the hypothalamic-pituitary-adrenal axis.
机构信息
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.
Department of Acupuncture and Moxibustion, the Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China.
出版信息
J Tradit Chin Med. 2023 Oct;43(5):944-954. doi: 10.19852/j.cnki.jtcm.20230727.001.
OBJECTIVE
To verify the hypothesis that electroacupuncture inhibits the hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis regulating the expression of glial fibrillary acidic protein (GFAP) in the hippocampus of acute myocardial ischemia (AMI) rats.
METHODS
Sixty-six healthy male Sprague-Dawley rats were randomly divided into five groups: Sham, AMI (Model), electroacupuncture at Shenmen (HT7)-Tongli (HT5) segment (EA), non-acupoint electroacupuncture (Control), and Model + corticosterone (Model + CORT). AMI was induced occlusion of the left anterior descending coronary artery, followed by 3 d of electroacupuncture at Shenmen (HT7)-Tongli (HT5) segment. In the Control group, electroacupuncture was applied at points lying 5 and 10 mm from the base of the tail. The AMI + CORT group was injected with CORT (20 mg/kg) in saline. Hemorheology, electrocardiography (ECG), hematoxylin and eosin staining, and expression of glycogen phosphorylase BB (GPBB) and heart-type fatty acid-binding protein (H-FABP) were used to assess cardiac function. The effects of adrenocorticotropic hormone (ACTH) and CORT were evaluated by enzyme-linked immunosorbent assay. Protein expression in the Sham and Model groups were screened by tandem mass tag-based quantitative proteomics analysis. Protein expression was evaluated by Western blotting (vimentin and GFAP) and immunofluorescence staining (GFAP).
RESULTS
Compared with the Sham group, the hemorheology indicators, heart rate, ECG-ST segment elevation, and GPBB and H-FABP levels were higher in Model rats. The EA group showed reductions in these indicators compared with the Model group. Similarly, in Model rats, the expression of ACTH and CORT were significantly increased compared with the Sham group. The EA group also showed reduced expression of ACTH and CORT. Importantly, proteomics analysis showed that vimentin was differentially expressed in Model rats. Compared with the Sham group, vimentin and GFAP expression in the hippocampus was increased in the Model group but decreased in the AMI + EA group. Additionally, intraperitoneal injection of CORT aggravated the expression of GPBB, H-FABP and GFAP.
CONCLUSIONS
Our results suggested that electroacupuncture may protect against cardiac injury induced by AMI through regulation of HPA axis hyperactivity, and that hippocampal GFAP may play an important role in the regulation.
目的
验证电针对调节急性心肌缺血（AMI）大鼠海马胶质纤维酸性蛋白（GFAP）表达的下丘脑-垂体-肾上腺（HPA）轴活性亢进的假说。
方法
将 66 只健康雄性 Sprague-Dawley 大鼠随机分为 5 组：假手术（Sham）组、AMI（模型）组、电针心经-神门（HT7-TL5）段（EA）组、非经非穴电针（对照）组、模型+皮质酮（模型+CORT）组。AMI 组通过结扎左前降支冠状动脉诱导 AMI，随后进行 3 d 的电针心经-神门（HT7-TL5）段。对照（Control）组，在距尾根 5 和 10 mm 处进行电针。模型+CORT 组，以生理盐水注射皮质酮（20 mg/kg）。血流变学、心电图（ECG）、苏木精-伊红染色和糖原磷酸化酶 BB（GPBB）和心脏型脂肪酸结合蛋白（H-FABP）的表达用于评估心功能。通过酶联免疫吸附测定（ELISA）评估促肾上腺皮质激素（ACTH）和皮质酮的作用。通过串联质量标签定量蛋白质组学分析筛选 Sham 和模型组的蛋白质表达。通过 Western 印迹（波形蛋白和 GFAP）和免疫荧光染色（GFAP）评估蛋白表达。
结果
与 Sham 组相比，模型组的血液流变学指标、心率、心电图-ST 段抬高和 GPBB 和 H-FABP 水平升高。EA 组与模型组相比，这些指标降低。同样，在模型组中，ACTH 和皮质酮的表达明显高于 Sham 组。EA 组也显示 ACTH 和皮质酮的表达减少。重要的是，蛋白质组学分析表明模型组中波形蛋白差异表达。与 Sham 组相比，模型组海马中波形蛋白和 GFAP 的表达增加，但在 AMI+EA 组中减少。此外，腹腔内注射皮质酮加重了 GPBB、H-FABP 和 GFAP 的表达。
结论
我们的结果表明，电针可能通过调节 HPA 轴活性亢进来保护 AMI 引起的心脏损伤，而海马 GFAP 可能在调节中发挥重要作用。
Zotero 插件