Yan Qiong, Jia Guoyun, Tan Wenjing, Tian Ran, Zheng Xiaochen, Feng Junming, Luo Xiaoqin, Si Binfan, Li Xin, Huang Kebing, Wang Meinan, Chen Xianming, Ren Yong, Yang Suizhuang, Zhou Xinli
Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China.
Department of Plant Pathology, Washington State University, Pullman, WA, United States.
Front Plant Sci. 2023 Aug 28;14:1232897. doi: 10.3389/fpls.2023.1232897. eCollection 2023.
INTRODUCTION: Stripe rust is a global disease of wheat. Identification of new resistance genes is key to developing and growing resistant varieties for control of the disease. Wheat line PI 660122 has exhibited a high level of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 239 recombinant inbred lines (RILs) was developed from a cross between PI 660122 and an elite Chinese cultivar Zhengmai 9023. METHODS: The RIL population was phenotyped for stripe rust response in three field environments and genotyped with the Wheat 15K single-nucleotide polymorphism (SNP) array. RESULTS: A total of nine quantitative trait loci (QTLs) for stripe rust resistance were mapped to chromosomes 1B (one QTL), 2B (one QTL), 4B (two QTLs), 4D (two QTLs), 6A (one QTL), 6D (one QTL), and 7D (one QTL), of which seven QTLs were stable and designated as , , , , , , and . was a major all-stage resistance QTL explaining the highest percentage (10.67%-20.97%) of the total phenotypic variation and was mapped to a 12.15-cM interval flanked by SNP markers and on chromosome 4DS. DISCUSSION: The QTL and their linked SNP markers in this study can be used in wheat breeding to improve resistance to stripe rust. In addition, 26 lines were selected based on stripe rust resistance and agronomic traits in the field for further selection and release of new cultivars.
引言：条锈病是小麦的一种全球性病害。鉴定新的抗性基因是培育和种植抗病品种以控制该病的关键。小麦品系PI 660122在十多年来一直表现出高水平的条锈病抗性。然而，该品系条锈病抗性的遗传特性尚未得到研究。通过PI 660122与中国优良品种郑麦9023杂交，构建了一套包含239个重组自交系（RIL）的群体。 方法：对该RIL群体在三个田间环境下的条锈病反应进行表型鉴定，并用小麦15K单核苷酸多态性（SNP）芯片进行基因分型。 结果：共定位到9个条锈病抗性数量性状位点（QTL），分别位于1B染色体（1个QTL）、2B染色体（1个QTL）、4B染色体（2个QTL）、4D染色体（2个QTL）、6A染色体（1个QTL）、6D染色体（1个QTL）和7D染色体（1个QTL）上，其中7个QTL稳定，分别命名为 、 、 、 、 、 、 。 是一个主要的全生育期抗性QTL，解释了总表型变异的最高比例（10.67%-20.97%），定位在4DS染色体上，侧翼为SNP标记 和 的12.15厘摩区间内。 讨论：本研究中的QTL及其连锁的SNP标记可用于小麦育种，以提高对条锈病的抗性。此外，根据田间条锈病抗性和农艺性状选择了26个品系，用于进一步筛选和新品种的发布。
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