双峰驼聚集的淋巴滤泡区和派伊尔氏斑的蛋白质组表达图谱相似。

Similar proteome expression profiles of the aggregated lymphoid nodules area and Peyer's patches in Bactrian camel.

机构信息

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, China.

The Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA, 5371, Australia.

出版信息

BMC Genomics. 2023 Oct 11;24(1):608. doi: 10.1186/s12864-023-09715-5.

DOI:10.1186/s12864-023-09715-5

PMID:37821839

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10568864/

Abstract

BACKGROUND

The presence of Aggregated Lymphoid Nodules Area (ALNA) is a notable anatomical characteristic observed in the abomasum of Bactrian camels. This area is comprised of two separate regions, namely the Reticular Mucosal Folds Region (RMFR) and the Longitudinal Mucosal Folds Region (LMFR). The histological properties of ALNA exhibit significant similarities to those of Peyer's patches (PPs) found in the gastrointestinal system. The functional characteristics of ALNA were examined in relation to mucosal immunity in the gastrointestinal system.

RESULTS

We used iTRAQ-based proteomic analysis on twelve Bactrian camels to measure the amount of proteins expressed in ALNA. In the experiment, we sampled the RMFR and LMFR separately from the ALNA and compared their proteomic quantification results with samples from the PPs. A total of 1253 proteins were identified, among which 39 differentially expressed proteins (DEPs) were found between RMFR and PPs, 33 DEPs were found between LMFR and PPs, and 22 DEPs were found between LMFR and RMFR. The proteins FLNA, MYH11, and HSPB1 were chosen for validation using the enzyme-linked immunosorbent assay (ELISA), and the observed expression profiles were found to be in agreement with the results obtained from the iTRAQ study. The InnateDB database was utilized to get data pertaining to immune-associated proteins in ALNA. It was observed that a significant proportion, specifically 76.6%, of these proteins were found to be associated with the same orthogroups as human immune-related genes. These proteins are acknowledged to be associated with a diverse range of functions, encompassing the uptake, processing and presentation of antigens, activation of lymphocytes, the signaling pathways of T-cell and B-cell receptors, and the control of actin polymerization.

CONCLUSIONS

The experimental results suggest that there are parallels in the immune-related proteins found in ALNA and PPs. Although there are variations in the structures of LMFR and RMFR, the proteins produced in both structures exhibit a high degree of similarity and perform comparable functions in the context of mucosal immune responses.

摘要

背景

在双峰驼的皱胃中，聚集的淋巴小结区域（ALNA）是一个显著的解剖学特征。该区域由两个独立的区域组成，即网状黏膜褶皱区（RMFR）和纵行黏膜褶皱区（LMFR）。ALNA 的组织学特性与胃肠道中的派尔集合淋巴结（PPs）非常相似。ALNA 的黏膜免疫功能特征在胃肠道中进行了研究。

结果

我们使用基于 iTRAQ 的蛋白质组学分析对 12 只双峰驼进行了研究，以测量 ALNA 中表达的蛋白质数量。在实验中，我们分别从 ALNA 中取样 RMFR 和 LMFR，并将其蛋白质组定量结果与 PPs 的样本进行比较。共鉴定出 1253 种蛋白质，其中 39 种差异表达蛋白（DEPs）在 RMFR 和 PPs 之间，33 种 DEPs 在 LMFR 和 PPs 之间，22 种 DEPs 在 LMFR 和 RMFR 之间。选择 FLNA、MYH11 和 HSPB1 三种蛋白质进行酶联免疫吸附试验（ELISA）验证，观察到的表达谱与 iTRAQ 研究结果一致。利用 InnateDB 数据库获取与 ALNA 中免疫相关蛋白相关的数据。结果表明，其中 76.6%的蛋白与人类免疫相关基因的相同同源基因具有显著的相关性。这些蛋白与多种功能相关，包括抗原的摄取、加工和呈递，淋巴细胞的激活，T 细胞和 B 细胞受体的信号通路，以及肌动蛋白聚合的控制。