Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture , Nanjing, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology , Nanjing, China.
J Virol. 2023 Nov 30;97(11):e0095823. doi: 10.1128/jvi.00958-23. Epub 2023 Oct 17.
As an emerging porcine enteropathogenic coronavirus that has the potential to infect humans, porcine deltacoronavirus (PDCoV) is receiving increasing attention. However, no effective commercially available vaccines against this virus are available. In this work, we designed a spike (S) protein and receptor-binding domain (RBD) trimer as a candidate PDCoV subunit vaccine. We demonstrated that S protein induced more robust humoral and cellular immune responses than the RBD trimer in mice. Furthermore, the protective efficacy of the S protein was compared with that of inactivated PDCoV vaccines in piglets and sows. Of note, the immunized piglets and suckling pig showed a high level of NAbs and were associated with reduced virus shedding and mild diarrhea, and the high level of NAbs was maintained for at least 4 months. Importantly, we demonstrated that S protein-based subunit vaccines conferred significant protection against PDCoV infection.
作为一种新兴的具有感染人类潜力的猪肠道致病性冠状病毒，猪德尔塔冠状病毒（PDCoV）受到越来越多的关注。然而，目前尚无针对该病毒的有效商业疫苗。在这项工作中，我们设计了一种刺突（S）蛋白和受体结合域（RBD）三聚体作为 PDCoV 亚单位候选疫苗。我们证明，S 蛋白在小鼠中诱导的体液和细胞免疫应答强于 RBD 三聚体。此外，我们还比较了 S 蛋白与猪 PDCoV 灭活疫苗在仔猪和母猪中的保护效力。值得注意的是，免疫仔猪和哺乳仔猪表现出高滴度的中和抗体，且与病毒脱落减少和轻度腹泻有关，中和抗体的高水平至少维持 4 个月。重要的是，我们证明了基于 S 蛋白的亚单位疫苗对 PDCoV 感染具有显著的保护作用。
