Lan Yanru, Yan Zhichen, Duan Tingyu
State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China.
Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou 730020, China.
J Fungi (Basel). 2023 Oct 30;9(11):1062. doi: 10.3390/jof9111062.
Luobuma ( and ) is widely cultivated for environmental conservation, medicinal purposes and the textile industry. In 2018, a severe leaf spot disease that attacked the leaves of Luobuma was observed in plants cultivated in Yuzhong County, Gansu Province, China. Symptoms of the disease appeared as white or off-white spots surrounded by brown margins on the leaves of . The spots expanded and covered a large area of the leaf, presenting as "cankers" with progression of the disease, leading to leaf death. The initial symptoms of the disease on were similar to the symptoms of , with a larger disease spot than and the spot was black and thicker. The aim of this study was to identify the fungal species and evaluate the effectiveness of fungicides (hymexazol and zhongshengmycin) against the pathogen in vitro. The fungi species that caused the new disease was identified as based on the morphological characteristics, pathogenicity tests, and phylogenetic analysis of the internal transcribed spacer () region, glyceraldehyde 3-phosphate dehydrogenase (), translation elongation factor 1-alpha () and the histone 3 () gene sequences. The findings showed that hymexazol fungicide can be used to control leaf spot disease. This is the first report on Luobuma leaf spot disease caused by in China.
罗布麻被广泛种植用于环境保护、药用和纺织工业。2018年,在中国甘肃省榆中县种植的罗布麻植株上观察到一种严重的叶斑病,该病侵袭了罗布麻的叶片。病害症状表现为叶片上出现白色或灰白色斑点,周围有褐色边缘。随着病情发展,病斑扩大并覆盖叶片的大片区域,呈现为“溃疡”,导致叶片死亡。该病最初在[此处原文可能有误,推测应为罗布麻]上的症状与[此处原文可能有误,推测应为某种已知病害]的症状相似,但病斑比[此处原文可能有误,推测应为某种已知病害]的更大,且病斑为黑色且更厚。本研究的目的是鉴定引起该病害的真菌种类,并在体外评估杀菌剂(噁霉灵和中生菌素)对该病原菌的防治效果。基于形态特征、致病性测试以及对内部转录间隔区(ITS)、甘油醛-3-磷酸脱氢酶(GAPDH)、翻译延伸因子1-α(TEF1-α)和组蛋白3(HIS3)基因序列的系统发育分析,将引起这种新病害的真菌种类鉴定为[此处原文可能有误,推测应为某种真菌名称]。研究结果表明,噁霉灵杀菌剂可用于防治叶斑病。这是中国关于由[此处原文可能有误,推测应为某种真菌名称]引起的罗布麻叶斑病的首次报道。