Key Laboratory of Macromolecular Synthesis and Functionalization (MOE), ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.
College of Pharmacy, Hangzhou Normal University, Hangzhou, 311121, China.
Mikrochim Acta. 2024 Jan 19;191(2):107. doi: 10.1007/s00604-023-06150-x.
A novel strategy based on gradient porous hollow fiber membrane (GPF) is proposed for the modular assembly of enzyme-nanozyme cascade systems. The porous structure of GPF provided sufficient specific surface area, while the gradient structure effectively minimized the leaching of enzymes and nanozymes. To enhance stability, we prepared and immobilized metal-organic framework (MOF) nanozymes, resulting in the fabrication of GPF-MOF with excellent stability and reusability for colorimetric HO detection. To improve specificity and expand the detection range, micro-crosslinked natural enzymes were modularly assembled, using glucose oxidase as the model enzyme. The assembled system, GPF-mGOx@MOF, achieved a low detection limit of 0.009 mM and a linear range of 0.2 to 11 mM. The sensor retained 87.2% and 80.7% of initial activity after being stored for 49 days and 9 recycles, respectively. Additionally, the reliability of the biosensor was validated through glucose determination of human blood and urine samples, yielding comparable results to a commercial glucose meter.
提出了一种基于梯度多孔空心纤维膜（GPF）的新型策略，用于酶-纳米酶级联系统的模块化组装。GPF 的多孔结构提供了足够的比表面积，而梯度结构则有效地减少了酶和纳米酶的浸出。为了提高稳定性，我们制备并固定了金属有机骨架（MOF）纳米酶，从而制造出具有出色稳定性和可重复使用性的用于比色 HO 检测的 GPF-MOF。为了提高特异性和扩展检测范围，使用葡萄糖氧化酶作为模型酶，对微交联天然酶进行了模块化组装。组装的系统 GPF-mGOx@MOF 实现了低至 0.009 mM 的检测限和 0.2 至 11 mM 的线性范围。该传感器在储存 49 天和 9 次循环后，初始活性分别保留了 87.2%和 80.7%。此外，通过对人血和尿样中的葡萄糖进行测定，验证了生物传感器的可靠性，得到的结果与商业葡萄糖计相当。
