Department of Mechanical Biosystems, Faculty of Agriculture, Urmia University, Urmia, Iran.
Department of Agronomy and Plant Breeding, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
Sci Rep. 2024 Jan 23;14(1):1995. doi: 10.1038/s41598-023-50948-x.
This study proposed a quick and reliable thermography-based method for detection of healthy potato tubers from those with dry rot disease and also determination of the level of disease development. The dry rot development inside potato tubers was classified based on the Wiersema Criteria, grade 0 to 3. The tubers were heated at 60 and 90 °C, and then thermal images were taken 10, 25, 40, and 70 s after heating. The surface temperature of the tubers was measured to select the best treatment for thermography, and the treatment with the highest thermal difference in each class was selected. The results of variance analysis of tuber surface temperature showed that tuber surface temperature was significantly different due to the severity of disease development inside the tuber. Total of 25 thermal images were prepared for each class, and then Otsu's threshold method was employed to remove the background. Their histograms were extracted from the red, green, and blue surfaces, and, finally, six features were extracted from each histogram. Moreover, the co-occurrence matrix was extracted at four angles from the gray level images and five features were extracted from each co-occurrence matrix. Totally, each thermograph was described by 38 features. These features were used to implement the artificial neural networks and the support vector machine in order to classify and diagnose the severity of the disease. The results showed that the sensitivity of the models in the diagnosis of healthy tubers was 96 and 100%, respectively. The overall accuracy of the models in detecting the severity of tuber tissue destruction was 93 and 97%, respectively. The proposed methodology as an accurate, nondestructive, fast, and applicable system reduces the potato loss by rapid detection of the disease of the tubers.
本研究提出了一种基于热成像的快速可靠方法,用于从健康的马铃薯块茎中检测出患有干腐病的马铃薯块茎,并确定干腐病的发展程度。根据 Wiersema 标准,将马铃薯块茎内部的干腐病发展程度分为 0 到 3 级。将块茎加热至 60 和 90°C,然后在加热后 10、25、40 和 70 秒拍摄热图像。测量块茎的表面温度以选择最佳的热成像处理方法,并选择每个级别中温差最大的处理方法。块茎表面温度的方差分析结果表明,由于块茎内部干腐病发展的严重程度,块茎表面温度存在显著差异。为每个级别准备了总共 25 张热图像,然后采用 Otsu 阈值法去除背景。从红色、绿色和蓝色表面提取它们的直方图,最后从每个直方图中提取六个特征。此外,从灰度图像的四个角度提取共生矩阵,并从每个共生矩阵中提取五个特征。总共,每个热图像由 38 个特征描述。这些特征用于实现人工神经网络和支持向量机,以对疾病的严重程度进行分类和诊断。结果表明,模型在诊断健康块茎时的灵敏度分别为 96%和 100%。模型检测块茎组织破坏严重程度的总准确率分别为 93%和 97%。该方法作为一种准确、无损、快速且适用的系统,通过快速检测块茎的病害,减少了马铃薯的损失。