Department of Medical Laboratory Science, College of Health Sciences, Salale University, Oromia, Ethiopia.
Department of Microbiology, Immunology, and parasitology, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia.
PLoS One. 2024 Mar 7;19(3):e0289586. doi: 10.1371/journal.pone.0289586. eCollection 2024.
Burns are one of the most common forms of trauma globally. P. aeruginosa plays a prominent role as an etiological agent among burn patients. There is a paucity of information about the prevalence and antimicrobial resistance patterns of P. aeruginosa among burn patients in Ethiopia. Hence, this study was designed to assess the prevalence and antimicrobial-resistant patterns of P. aeruginosa among burn patients attending Yekatit 12 Hospital Medical College in Addis Ababa, Ethiopia.
Hospital-based cross-sectional study was conducted at Yekatit 12 Hospital Medical College among burn patients from November 2020 to April 2021. Identification of P. aeruginosa was performed using Culture, Biochemical tests, and, Gram staining. Antimicrobial resistance testing was done using the Kirby-Bauer disc diffusion method. Logistic regression was computed to determine associated factors.
From 210 burn wound cultures, 27 (12.86%) were found positive for P. aeruginosa. All the isolates showed greater than 70% susceptibility to the tested antibiotics except Gentamycin, Ceftazidime, and, Ciprofloxacin. In addition, 33.33% of P. aeruginosa isolates were multidrug-resistant. Admission type, Hospital stay time and Total body surface area (TBSA) had a statistically significant association (all with P-value <0.05) with the acquisition of P. aeruginosa infection.
Overall, the prevalence of P. aeruginosa isolates among burn patients is almost 13%. Most P. aeruginosa isolates were sensitive to Imipenem, while they were most resistant to Gentamycin. One-third of P. aeruginosa were multidrug-resistant. This suggests the need to monitor the treatment of infection with the pathogen to limit the possibility of the emergence of multidrug-resistant isolates in burn centers.
烧伤是全球最常见的创伤形式之一。铜绿假单胞菌在烧伤患者中作为一种病因菌起着突出的作用。关于埃塞俄比亚烧伤患者中铜绿假单胞菌的流行情况和抗生素耐药模式的信息很少。因此，本研究旨在评估在埃塞俄比亚亚的斯亚贝巴 Yekatit 12 医院医学院就诊的烧伤患者中铜绿假单胞菌的流行情况和抗生素耐药模式。
这是一项在 Yekatit 12 医院医学院进行的烧伤患者的医院横断面研究，时间为 2020 年 11 月至 2021 年 4 月。使用培养、生化试验和革兰氏染色来鉴定铜绿假单胞菌。使用 Kirby-Bauer 纸片扩散法进行抗生素耐药性检测。使用逻辑回归来确定相关因素。
从 210 份烧伤创面培养物中，有 27 份（12.86%）培养出铜绿假单胞菌阳性。所有分离株对所测试的抗生素的敏感性均大于 70%，除了庆大霉素、头孢他啶和环丙沙星。此外，33.33%的铜绿假单胞菌分离株为多重耐药菌。入院类型、住院时间和总体表面积（TBSA）与铜绿假单胞菌感染的获得具有统计学显著关联（均 P 值<0.05）。
总体而言，烧伤患者中铜绿假单胞菌分离株的流行率约为 13%。大多数铜绿假单胞菌分离株对亚胺培南敏感，而对庆大霉素最耐药。三分之一的铜绿假单胞菌为多重耐药菌。这表明需要监测感染该病原体的治疗情况，以限制烧伤中心出现多重耐药分离株的可能性。
