Yang Jun, Ahmed Waqar, Zhang Jinhao, Gao Shunyu, Wang Zhenji, Yang Haiyan, Bai Xuehui, Luo Kai, Xu Chengdong, Ji Guanghai
College of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong 675000, China.
State Key Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Agricultural University, Kunming 650201, China.
Life (Basel). 2024 Feb 20;14(3):285. doi: 10.3390/life14030285.
Leaf-blight disease caused by the is an emerging problem in production in China. Symptoms of leaf blight were observed on seedlings of cultivated in a nursery in Ruili City, Yunnan Province, China. In this study, we isolated the sp. associated with leaf-blight disease of from the diseased seedlings. A pathogenicity test was performed to fulfill Koch's postulates to confirm the pathogenicity of isolated strains and identified using morphological and molecular techniques. The results revealed that all four isolated sp. isolates (DHRL-01~04) produced typical blight symptoms followed by marginal necrosis of leaves on the plants. On the PDA medium, the fungal colony appeared as a white to purplish color with cottony mycelium growth. Microconidia are oval-shaped, whereas macroconidia are sickle-shaped, tapering at both ends with 2-4 septations. The phylogenetic trees were construed based on internal transcribed spacer (), translation elongation factor (), and RNA polymerase subunit genes and genes, respectively, and blasted against the NCBI database for species confirmation. Based on the NCBI database's blast results, the isolates showed that more than 99% identify with . To our knowledge, this is the first comprehensive report on the identification of as the causal agent of leaf blight in Yunnan Province, China, based on morphological and molecular characteristics.
由[病原菌名称未给出]引起的叶枯病是中国[作物名称未给出]生产中一个新出现的问题。在中国云南省瑞丽市一个苗圃中栽培的[作物名称未给出]幼苗上观察到了叶枯病症状。在本研究中,我们从患病幼苗中分离出了与[作物名称未给出]叶枯病相关的[病原菌名称未给出]菌株。进行了致病性测试以满足科赫法则,确认分离菌株的致病性,并使用形态学和分子技术进行鉴定。结果表明,所有四个分离的[病原菌名称未给出]菌株(DHRL - 01~04)在[作物名称未给出]植株上都产生了典型的叶枯症状,随后叶片边缘坏死。在PDA培养基上,真菌菌落呈白色至紫色,有棉絮状菌丝生长。小分生孢子呈椭圆形,而大分生孢子呈镰刀形,两端逐渐变细,有2 - 4个隔膜。分别基于内转录间隔区(ITS)、翻译延伸因子(TEF)以及RNA聚合酶亚基基因[基因名称未给出]和[基因名称未给出]构建系统发育树,并与NCBI数据库进行比对以确认物种。根据NCBI数据库的比对结果,这些分离株显示与[病原菌名称未给出]的相似度超过99%。据我们所知,这是基于形态学和分子特征首次全面报道[病原菌名称未给出]是中国云南省[作物名称未给出]叶枯病的致病因子。