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不同光照和菌根条件下工业大麻幼叶的抗氧化反应及酚类物质含量

Antioxidative Response and Phenolic Content of Young Industrial Hemp Leaves at Different Light and Mycorrhiza.

作者信息

Varga Ivana, Kristić Marija, Lisjak Miroslav, Tkalec Kojić Monika, Iljkić Dario, Jović Jurica, Kristek Suzana, Markulj Kulundžić Antonela, Antunović Manda

机构信息

Department of Plant Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia.

Department of Agroecology and Environment Protection, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia.

出版信息

Plants (Basel). 2024 Mar 14;13(6):840. doi: 10.3390/plants13060840.

Abstract

Due to the increasing presence of industrial hemp ( L.) and its multiple possibilities of use, the influence of different light and several biopreparations based on beneficial fungi and bacteria on hemp's morphological and physiological properties were examined. Different biopreparations and their combinations were inoculated on hemp seed and/or substrate and grown under blue and white light. A completely randomized block design was conducted in four replications within 30 days. For biopreparation treatment, vesicular arbuscular mycorrhiza (VAM) in combination with and spp. were inoculated only on seed or both on seed and in the substrate. Generally, the highest morphological parameters (stem, root and plant length) were recorded on plants in white light and on treatment with applied spp., both on seed and substrate. Blue light negatively affected biopreparation treatments, resulting in lower values of all morphological parameters compared to control. Leaves pigments were higher under blue light, as compared to the white light. At the same time, 1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), flavonoids, total flavanol content and phenolic acids were not influenced by light type. Biopreparation treatments did not significantly influence the leaves' pigments content (Chl a, Chl b and Car), nor the phenolic and flavanol content.

摘要

由于工业大麻(L.)的种植日益增加及其多种使用可能性,研究了不同光照以及几种基于有益真菌和细菌的生物制剂对大麻形态和生理特性的影响。将不同的生物制剂及其组合接种到大麻种子和/或基质上,并在蓝光和白光下种植。在30天内进行了四次重复的完全随机区组设计。对于生物制剂处理,丛枝菌根(VAM)与和 spp. 的组合仅接种在种子上,或同时接种在种子和基质中。一般来说,在白光下以及在种子和基质上均施用 spp. 处理的植株记录到最高的形态参数(茎、根和植株长度)。蓝光对生物制剂处理有负面影响,与对照相比,所有形态参数的值均较低。与白光相比,蓝光下叶片色素含量更高。同时,1,1-二苯基-2-苦基肼(DPPH)、铁还原抗氧化能力(FRAP)、黄酮类化合物、总黄烷醇含量和酚酸不受光照类型的影响。生物制剂处理对叶片色素含量(叶绿素a、叶绿素b和类胡萝卜素)以及酚类和黄烷醇含量没有显著影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7edf/10976054/7fd62229273e/plants-13-00840-g001.jpg

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