肠道微生物组与冠状动脉疾病严重程度的改变。

Alterations in the gut mycobiome with coronary artery disease severity.

机构信息

Department of Cardiac Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China; Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China.

Heart Center and Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China; Department of Cardiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China.

出版信息

EBioMedicine. 2024 May;103:105137. doi: 10.1016/j.ebiom.2024.105137. Epub 2024 May 3.

DOI:10.1016/j.ebiom.2024.105137

PMID:38703606

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11087906/

Abstract

BACKGROUND

Coronary artery disease (CAD) is a prevalent cardiovascular condition, and numerous studies have linked gut bacterial imbalance to CAD. However, the relationship of gut fungi, another essential component of the intestinal microbiota, with CAD remains poorly understood.

METHODS

In this cross-sectional study, we analyzed fecal samples from 132 participants, split into 31 healthy controls and 101 CAD patients, further categorized into stable CAD (38), unstable angina (41), and acute myocardial infarction (22) groups. We conducted internal transcribed spacer 1 (ITS1) and 16S sequencing to examine gut fungal and bacterial communities.

FINDINGS

Based on ITS1 analyses, Ascomycota and Basidiomycota were the dominant fungal phyla in all the groups. The α diversity of gut mycobiome remained unaltered among the control group and CAD subgroups; however, the structure and composition of the mycobiota differed significantly with the progression of CAD. The abundances of 15 taxa gradually changed with the occurrence and progression of the disease and were significantly correlated with major CAD risk factor indicators. The mycobiome changes were closely linked to gut microbiome dysbiosis in patients with CAD. Furthermore, disease classifiers based on gut fungi effectively identified subgroups with different degrees of CAD. Finally, the FUNGuild analysis further categorized these fungi into distinct ecological guilds.

INTERPRETATION

In conclusion, the structure and composition of the gut fungal community differed from healthy controls to various subtypes of CAD, revealing key fungi taxa alterations linked to the onset and progression of CAD. Our study highlights the potential role of gut fungi in CAD and may facilitate the development of novel biomarkers and therapeutic targets for CAD.

FUNDING

This work was supported by the grants from the National Natural Science Foundation of China (No. 82170302, 92168117, 82370432), National clinical key specialty construction project- Cardiovascular Surgery, the Reform and Development Program of Beijing Institute of Respiratory Medicine (No. Ggyfz202417, Ggyfz202308), the Beijing Natural Science Foundation (No. 7222068); and the Clinical Research Incubation Program of Beijing Chaoyang Hospital Affiliated to Capital Medical University (No. CYFH202209).

摘要

背景

冠心病（CAD）是一种常见的心血管疾病，许多研究将肠道细菌失衡与 CAD 联系起来。然而，肠道真菌（肠道微生物群的另一个重要组成部分）与 CAD 的关系仍知之甚少。

方法

在这项横断面研究中，我们分析了来自 132 名参与者的粪便样本，分为 31 名健康对照组和 101 名 CAD 患者，进一步分为稳定型 CAD（38 例）、不稳定型心绞痛（41 例）和急性心肌梗死（22 例）组。我们进行了内部转录间隔区 1（ITS1）和 16S 测序，以检查肠道真菌和细菌群落。

结果

基于 ITS1 分析，子囊菌门和担子菌门是所有组中主要的真菌门。在对照组和 CAD 亚组中，肠道真菌组的α多样性保持不变；然而，随着 CAD 的进展，真菌群的结构和组成差异显著。随着疾病的发生和发展，15 个分类群的丰度逐渐发生变化，并且与主要 CAD 风险因素指标显著相关。真菌组的变化与 CAD 患者的肠道微生物群失调密切相关。此外，基于肠道真菌的疾病分类器有效地识别了具有不同 CAD 程度的亚组。最后，FUNGuild 分析进一步将这些真菌分为不同的生态类群。

结论

总之，从健康对照组到不同类型的 CAD，肠道真菌群落的结构和组成存在差异，揭示了与 CAD 发病和进展相关的关键真菌分类群的改变。我们的研究强调了肠道真菌在 CAD 中的潜在作用，并可能有助于为 CAD 开发新的生物标志物和治疗靶点。

资助

本工作得到了国家自然科学基金（No. 82170302、92168117、82370432）、国家临床重点专科建设项目-心血管外科、北京呼吸疾病研究所改革与发展项目（No. Ggyfz202417、Ggyfz202308）、北京市自然科学基金（No. 7222068）和首都医科大学附属北京朝阳医院临床研究孵化计划（No. CYFH202209）的支持。