State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmaceutical Sciences, Guizhou Medical University, Guizhou 561113, China; The High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou Province, School of Pharmaceutical Sciences, Guizhou Medical University, Guizhou 561113, China.
The First People's Hospital of Guiyang, Guizhou 550002, China.
Colloids Surf B Biointerfaces. 2024 Sep;241:114017. doi: 10.1016/j.colsurfb.2024.114017. Epub 2024 Jun 7.
Inspired by the "natural camouflage" strategy, cell-based biomimetic drug delivery systems (BDDS) have shown great potential in cancer therapy. Red blood cell (RBC) delivery vehicles and red blood cell membrane (RBCm)-camouflaged vehicles were commonly used strategies for drug delivery. We prepared shikonin-encapsulated PLGA nanoparticles (PLGA/SK) with different surface charges to obtain both RBC delivery and RBCm-camouflaged PLGA NPs. The physicochemical properties, in vivo circulation and antitumor effects of these biomimetic preparations were studied. Since the positive PLGA NPs may affect the morphology and function of RBCs, the biomimetic preparations prepared by the negative PLGA NPs showed better in vitro stability. However, positive PLGA NP-based biomimetic preparations exhibited longer circulation time and higher tumor region accumulation, leading to stronger anti-tumor effects. Meanwhile, the RBC delivery PLGA(+) NPs possessed better in vitro cytotoxicity, longer circulation time and higher tumor accumulation than RBCm-camouflaged PLGA(+) NPs. Collectively, RBC delivery vehicles possessed more potential than RBCm-camouflaged vehicles on drug delivery for tumor treatment, especially with positive NPs-loaded.
受“自然伪装”策略的启发，基于细胞的仿生药物传递系统（BDDS）在癌症治疗中显示出巨大的潜力。红细胞（RBC）传递载体和红细胞膜（RBCm）伪装载体是常用的药物传递策略。我们制备了具有不同表面电荷的载姜黄素的 PLGA 纳米颗粒（PLGA/SK），以获得 RBC 传递和 RBCm 伪装的 PLGA NPs。研究了这些仿生制剂的物理化学性质、体内循环和抗肿瘤作用。由于阳性 PLGA NPs 可能影响 RBC 的形态和功能，因此由阴性 PLGA NPs 制备的仿生制剂表现出更好的体外稳定性。然而，基于正 PLGA NP 的仿生制剂表现出更长的循环时间和更高的肿瘤区域积累，从而具有更强的抗肿瘤作用。同时，与 RBCm 伪装的 PLGA(+) NPs 相比，负载 RBC 的 PLGA(+) NPs 具有更好的体外细胞毒性、更长的循环时间和更高的肿瘤积累。总的来说，与 RBCm 伪装载体相比，RBC 传递载体在肿瘤治疗的药物传递方面具有更大的潜力，尤其是负载正 NPs 的载体。
