对高脂喂养与慢性心理应激联合作用的代谢反应。

Metabolic Responses to High-Fat Feeding and Chronic Psychological Stress Combination.

作者信息

Nemati Marzieh, Rostamkhani Fatemeh, Karbaschi Roxana, Zardooz Homeira

机构信息

Department of Physiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Endocrinology and Metabolism Research Center, Shiraz University of Medical Science, Shiraz, Iran.

出版信息

Endocrinol Diabetes Metab. 2024 Jul;7(4):e487. doi: 10.1002/edm2.487.

DOI:10.1002/edm2.487

PMID:38867382

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11168916/

Abstract

INTRODUCTION

High-fat diet (HFD) consumption and being exposed to daily psychological stress, common environmental factors in modern lifestyle, play an important role on metabolic disorders such as glucose homeostasis impairment. The aim of this study was to investigate the effects of high-fat diet (HFD) and psychological stress combination on metabolic response to chronic psychological stress in male rats.

METHOD

Male Wistar rats were divided into HFD, and normal diet (ND) groups and then into stress and nonstress subgroups. The diets were applied for 5 weeks, and psychological stress was induced for 7 consecutive days. Then, blood samples were taken to measure glucose, insulin, free fatty acids (FFA), and leptin and corticosterone concentrations. Subsequently, glucose-stimulated insulin release from pancreatic isolated islets was assessed.

RESULTS

HFD did not significantly change fasting plasma glucose, insulin and corticosterone levels, whereas increased plasma leptin (7.05 ± 0.33) and FFA (p < 0.01) levels and impaired glucose tolerance. Additionally, HFD and stress combination induced more profound glucose intolerance associated with increased plasma corticosterone (p < 0.01) and leptin (8.63 ± 0.38) levels. However, insulin secretion from isolated islets did not change in the presence of high-fat diet and/or stress.

CONCLUSION

HFD should be considered as an intensified factor of metabolic impairments caused by chronic psychological stress.

摘要

引言

食用高脂饮食（HFD）以及承受日常心理压力，这些现代生活方式中常见的环境因素，在诸如葡萄糖稳态受损等代谢紊乱中起着重要作用。本研究的目的是调查高脂饮食（HFD）与心理压力相结合对雄性大鼠慢性心理压力代谢反应的影响。

方法

将雄性Wistar大鼠分为高脂饮食组和正常饮食（ND）组，然后再分为应激和非应激亚组。饮食干预持续5周，连续7天诱导心理压力。然后采集血样以测量葡萄糖、胰岛素、游离脂肪酸（FFA）、瘦素和皮质酮浓度。随后，评估胰腺分离胰岛的葡萄糖刺激胰岛素释放情况。

结果

高脂饮食并未显著改变空腹血浆葡萄糖、胰岛素和皮质酮水平，然而却提高了血浆瘦素（7.05±0.33）和游离脂肪酸（p<0.01）水平，并损害了葡萄糖耐量。此外，高脂饮食与应激相结合导致更严重的葡萄糖不耐受，同时血浆皮质酮（p<0.01）和瘦素（8.63±0.38）水平升高。然而，在存在高脂饮食和/或应激的情况下，分离胰岛的胰岛素分泌并未改变。