Vahidi Akram, Khosravi Teymoor, Dastaviz Farzad, Sheikh Arabi Mehdi, Khosravi Ayyoob, Oladnabi Morteza
Student Research Committee, Golestan University of Medical Sciences, Gorgan 4934174611, Iran.
Department of Medical Nanotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan 4934174611, Iran.
Int J Ophthalmol. 2024 Jun 18;17(6):1028-1035. doi: 10.18240/ijo.2024.06.06. eCollection 2024.
To evaluate the effect of auraptene (AUR) treatment in forms of free and encapsulated in niosome nanoparticles by investigating the mRNA expression level of vascular endothelium growth factor (VEGF)-A and platelet-derived growth factors (PDGFs) in human retinal pigment epithelium (RPE) cell line.
Niosome nanocarriers were produced using two surfactants Span 60 and Tween 80. RPE cell line was treated with both free AUR and niosome-encapsulated. Optimum dosage of treatments was calculated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Expression of and , , , genes was measured after total RNA extraction and cDNA synthesis, using real-time polymerase chain reaction (RT-PCR).
The highest entrapment efficiency (EE) was achieved by Span 60:cholesterol (1:1) with 64.3%. The half maximal inhibitory concentration (IC) of free and niosome-encapsulated AUR were 38.5 and 27.78 µg/mL, respectively. Release study revealed that niosomal AUR had more gradual delivery to the cells. RT-PCR results showed reduced expression levels of , , , , and after treatment with both free and niosomal AUR.
Niosomal formulation of Span 60: cholesterol (1:1) is an effective drug delivery approach to transfer AUR to RPE cells. VEGF-A, PDGF-A, PDGF-B, PDGF-C, and PDGF-D are four angiogenic factors, inhibiting which by niosomal AUR may be effective in age-related macular degeneration.
通过研究人视网膜色素上皮（RPE）细胞系中血管内皮生长因子（VEGF）-A和血小板衍生生长因子（PDGFs）的mRNA表达水平，评估游离形式和包裹于脂质体纳米颗粒中的奥替普拉（AUR）的治疗效果。
使用两种表面活性剂司盘60和吐温80制备脂质体纳米载体。用游离AUR和脂质体包裹的AUR处理RPE细胞系。使用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法计算最佳治疗剂量。在提取总RNA并合成cDNA后，使用实时聚合酶链反应（RT-PCR）测量VEGF-A、PDGF-A、PDGF-B、PDGF-C和PDGF-D基因的表达。
司盘60:胆固醇（1:1）的包封率最高，为64.3%。游离AUR和脂质体包裹的AUR的半数最大抑制浓度（IC）分别为38.5和27.78 μg/mL。释放研究表明，脂质体AUR向细胞的递送更为缓慢。RT-PCR结果显示，用游离AUR和脂质体AUR处理后，VEGF-A、PDGF-A、PDGF-B、PDGF-C和PDGF-D的表达水平均降低。
司盘60:胆固醇（1:1）的脂质体制剂是将AUR转运至RPE细胞的有效药物递送方法。VEGF-A、PDGF-A、PDGF-B、PDGF-C和PDGF-D是四种血管生成因子，脂质体AUR抑制这些因子可能对年龄相关性黄斑变性有效。
