猪繁殖与呼吸综合征病毒 GP5 蛋白的铁蛋白蛋白纳米颗粒疫苗的研制。

Development of a Ferritin Protein Nanoparticle Vaccine with PRRSV GP5 Protein.

机构信息

National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.

出版信息

Viruses. 2024 Jun 20;16(6):991. doi: 10.3390/v16060991.

DOI:10.3390/v16060991

PMID:38932282

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11209462/

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) presents a significant threat to the global swine industry. The development of highly effective subunit nanovaccines is a promising strategy for preventing PRRSV variant infections. In this study, two different types of ferritin (Ft) nanovaccines targeting the major glycoprotein GP5, named GP5m-Ft and (Bp-IVp)-Ft, were constructed and evaluated as vaccine candidates for PRRSV. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) demonstrated that both purified GP5m-Ft and (Bp-IVp)-Ft proteins could self-assemble into nanospheres. A comparison of the immunogenicity of GP5m-Ft and (Bp-IVp)-Ft with an inactivated PRRSV vaccine in BALB/c mice revealed that mice immunized with GP5m-Ft exhibited the highest ELISA antibody levels, neutralizing antibody titers, the lymphocyte proliferation index, and IFN-γ levels. Furthermore, vaccination with the GP5m-Ft nanoparticle effectively protected piglets against a highly pathogenic PRRSV challenge. These findings suggest that GP5m-Ft is a promising vaccine candidate for controlling PRRS.

摘要

猪繁殖与呼吸综合征病毒（PRRSV）对全球养猪业构成重大威胁。开发高效的亚单位纳米疫苗是预防 PRRSV 变异感染的一种有前途的策略。在这项研究中，构建了两种针对主要糖蛋白 GP5 的不同类型的铁蛋白（Ft）纳米疫苗，分别命名为 GP5m-Ft 和（Bp-IVp）-Ft，并将其评估为 PRRSV 的候选疫苗。透射电子显微镜（TEM）和动态光散射（DLS）表明，两种纯化的 GP5m-Ft 和（Bp-IVp）-Ft 蛋白都可以自组装成纳米球。比较 GP5m-Ft 和（Bp-IVp）-Ft 与 PRRSV 灭活疫苗在 BALB/c 小鼠中的免疫原性，结果表明，用 GP5m-Ft 免疫的小鼠表现出最高的 ELISA 抗体水平、中和抗体滴度、淋巴细胞增殖指数和 IFN-γ 水平。此外，用 GP5m-Ft 纳米颗粒接种疫苗可有效保护仔猪免受高致病性 PRRSV 挑战。这些发现表明，GP5m-Ft 是控制 PRRS 的一种有前途的候选疫苗。