Gentner Clémence, Rogez Benoit, Robert Hadrien M L, Aggoun Anis, Tessier Gilles, Bon Pierre, Berto Pascal
Institut de la Vision, Sorbonne Université, CNRS-UMR 7210, Inserm-UMR S968, Paris 75012, France.
L2n, Université de technologie de Troyes, CNRS-UMR 7076, Troyes 10004, France.
ACS Nano. 2024 Jul 23;18(29):19247-19256. doi: 10.1021/acsnano.4c05152. Epub 2024 Jul 9.
Quantitative phase imaging enables precise and label-free characterizations of individual nano-objects within a large volume, without knowledge of the sample or imaging system. While emerging common path implementations are simple enough to promise a broad dissemination, their phase sensitivity still falls short of precisely estimating the mass or polarizability of vesicles, viruses, or nanoparticles in single-shot acquisitions. In this paper, we revisit the Zernike filtering concept, originally crafted for intensity-only detectors, with the aim of adapting it to wavefront imaging. We demonstrate, through numerical simulation and experiments based on high-resolution wavefront sensing, that a simple Fourier-plane add-on can significantly enhance phase sensitivity for subdiffraction objects─achieving over an order of magnitude increase (×12)─while allowing the quantitative retrieval of both intensity and phase. This advancement allows for more precise nano-object detection and metrology.
定量相成像能够在无需了解样品或成像系统的情况下，对大体积内的单个纳米物体进行精确的无标记表征。虽然新兴的共光路实现方式足够简单，有望得到广泛应用，但其相位灵敏度仍不足以在单次采集时精确估计囊泡、病毒或纳米颗粒的质量或极化率。在本文中，我们重新审视了最初为仅强度探测器设计的泽尼克滤波概念，旨在使其适用于波前成像。我们通过基于高分辨率波前传感的数值模拟和实验表明，一个简单的傅里叶平面附加装置可以显著提高亚衍射物体的相位灵敏度——实现超过一个数量级的提升（×12）——同时允许对强度和相位进行定量检索。这一进展使得纳米物体的检测和计量更加精确。
