Heindel Daniel W, Figueroa Acosta Dania M, Goff Marisa, Yengo Clauvis Kunkeng, Jan Muzafar, Liu Xiaomei, Wang Xiao-Hong, Petrova Mariya I, Zhang Mo, Sagar Manish, Barnette Phillip, Pandey Shilpi, Hessell Ann J, Chan Kun-Wei, Kong Xiang-Peng, Chen Benjamin K, Mahal Lara K, Bensing Barbara A, Hioe Catarina E
Divison of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
VA New York Harbor Healthcare System-Manhattan, New York, NY, USA.
iScience. 2024 Jun 27;27(8):110390. doi: 10.1016/j.isci.2024.110390. eCollection 2024 Aug 16.
Bacteria dysbiosis and its accompanying inflammation or compromised mucosal integrity is associated with an increased risk of HIV-1 transmission. However, HIV-1 may also bind bacteria or bacterial products to impact infectivity and transmissibility. This study evaluated HIV-1 interactions with bacteria through glycan-binding lectins. The Siglec-like lectin SLBR-N, a part of the fimbriae shrouding the bacteria surface that recognizes α2,3 sialyated -linked glycans, was noted for its ability to enhance HIV-1 infectivity in the context of cell-free infection and cell-to-cell transfer. Enhancing effects were recapitulated with -glycan-binding plant lectins, signifying the importance of -glycans. -glycan-binding bacterial lectins FimH and Msl had no effect. SLBR-N was demonstrated to capture and transfer infectious HIV-1 virions, bind to -glycans on HIV-1 Env, and increase HIV-1 resistance to neutralizing antibodies targeting different regions of Env. This study highlights the potential contribution of -glycan-binding lectins from commensal bacteria at the mucosa in promoting HIV-1 infection.
细菌生态失调及其伴随的炎症或黏膜完整性受损与HIV-1传播风险增加有关。然而，HIV-1也可能与细菌或细菌产物结合，从而影响其传染性和传播性。本研究通过聚糖结合凝集素评估了HIV-1与细菌的相互作用。Siglec样凝集素SLBR-N是覆盖细菌表面菌毛的一部分，可识别α2,3唾液酸化连接聚糖，在无细胞感染和细胞间转移的情况下，它具有增强HIV-1感染性的能力。聚糖结合植物凝集素也有类似增强作用，表明聚糖的重要性。聚糖结合细菌凝集素FimH和Msl则没有作用。研究表明，SLBR-N可捕获并转移具有传染性的HIV-1病毒粒子，与HIV-1包膜上的聚糖结合，并增加HIV-1对靶向包膜不同区域的中和抗体的抗性。本研究强调了共生细菌在黏膜处的聚糖结合凝集素在促进HIV-1感染方面的潜在作用。
