Qin Yanhong, Lu Shuhao, Wen Yi, Li Shaojian, Gao Suxia, Zhang Desheng, Li Xuemeng, Yang Jin, Gu Li, Li Mingjie, Wang Fei, Lu Chuantao
Institute of Plant Protection, Henan Academy of Agricultural Sciences, No. 116, Huanyuan Road, Jinshui District, Zhengzhou 450002, China.
College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Microorganisms. 2024 Aug 11;12(8):1643. doi: 10.3390/microorganisms12081643.
, a crucial medicinal plant native to China, is extensively cultivated across East Asia. We used high-throughput sequencing to identify viruses infecting with mosaic, leaf yellowing, and necrotic symptoms. A novel which we tentatively named "Rehmannia torradovirus virus" (ReTV), was identified. The complete sequences were obtained through reverse-transcription polymerase chain reaction (RT-PCR), 5' and 3' rapid amplification of cDNA ends, and Sanger sequencing. The amino acid sequence alignment between the ReTV-52 isolate and known species in the Pro-Pol and coat protein regions were 51.3-73.3% and 37.1-68.1%, respectively. Meanwhile, the amino acid sequence alignment between the ReTV-8 isolate and known species in the Pro-Pol and coat protein regions were 52.7-72.8% and 36.8-67.5%, respectively. The sequence analysis classified ten ReTV strains into two variants. The ReTV-52 genome has two RNA segments of 6939 and 4569 nucleotides, while that of ReTV-8 consists of two RNA segments containing 6889 and 4662 nucleotides. Sequence comparisons and phylogenetic analysis showed ReTV strains clustered within the exhibiting the closet relation to the squash chlorotic leaf spot virus. The RT-PCR results showed a 100% ReTV detection rate in all 60 samples. Therefore, ReTV should be classified as a novel species. ReTV is potentially dangerous to and necessitating monitoring this virus in the field.
地黄，一种原产于中国的重要药用植物，在东亚广泛种植。我们使用高通量测序来鉴定感染地黄出现花叶、叶片黄化和坏死症状的病毒。鉴定出一种新型病毒，我们暂定名为“地黄托拉病毒”（ReTV）。通过逆转录聚合酶链反应（RT-PCR）、5'和3' cDNA末端快速扩增以及桑格测序获得了完整序列。ReTV - 52分离株与已知病毒在蛋白酶-多聚酶区域和衣壳蛋白区域的氨基酸序列比对分别为51.3 - 73.3%和37.1 - 68.1%。同时，ReTV - 8分离株与已知病毒在蛋白酶-多聚酶区域和衣壳蛋白区域的氨基酸序列比对分别为52.7 - 72.8%和36.8 - 67.5%。序列分析将10个ReTV菌株分为两个变种。ReTV - 52基因组有两个RNA片段，分别为6939和4569个核苷酸，而ReTV - 8的基因组由两个分别包含6889和4662个核苷酸的RNA片段组成。序列比较和系统发育分析表明，ReTV菌株聚集在病毒属内，与南瓜褪绿叶斑病毒关系最为密切。RT-PCR结果显示，在所有60个地黄样本中ReTV的检测率为100%。因此，ReTV应被归类为一种新型病毒物种。ReTV对地黄有潜在危害，需要在田间对该病毒进行监测。
