Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University, UNESP, São José dos Campos, São Paulo, Brazil.
School of Dental Medicine, Lake Erie College of Osteopathic Medicine, Bradenton, FL, USA.
Sci Rep. 2024 Sep 5;14(1):20678. doi: 10.1038/s41598-024-70943-0.
The primary aim of this study was to investigate the impact of treatment with low-temperature plasma (LTP) for varying exposure durations on a multispecies cariogenic biofilm comprising C. albicans, L. casei, and S. mutans, as well as on single-species biofilms of L. casei and C. albicans, cultured on hydroxyapatite discs. Biofilms were treated with LTP-argon at a 10 mm distance for 30 s, 60 s, and 120 s. Chlorhexidine solution (0.12%) and NaCl (0.89%) were used as positive (PC) and negative controls (NC), respectively. Argon flow only was also used as gas flow control (F). Colony-forming units (CFU) recovery and confocal laser scanning microscopy (CLSM) were used to analyze biofilm viability. LTP starting at 30 s of application significantly reduced the viability of multispecies biofilms by more than 2 log in all treated samples (p < 0.0001). For single-species biofilms, L. casei showed a significant reduction compared to PC and NC of over 1 log at all exposure times (p < 0.0001). In the case of C. albicans biofilms, LTP treatment compared to PC and NC resulted in a significant decrease in bacterial counts when applied for 60 and 120 s (1.55 and 1.90 log10 CFU/mL, respectively) (p < 0.0001). A significant effect (p ≤ 0.05) of LTP in single-species biofilms was observed to start at 60 s of LTP application compared to F, suggesting a time-dependent effect of LTP for the single-species biofilms of C. albicans and L. casei. LTP is a potential mechanism in treating dental caries by being an effective anti-biofilm therapy of both single and multispecies cariogenic biofilms.
本研究的主要目的是研究不同暴露时间低温等离子体（LTP）治疗对包含白色念珠菌、干酪乳杆菌和变形链球菌的多物种致龋生物膜以及在羟基磷灰石盘上培养的干酪乳杆菌和白色念珠菌单物种生物膜的影响。生物膜用 10mm 距离的 LTP-氩气处理 30s、60s 和 120s。0.12%洗必泰溶液和 0.89%NaCl 分别用作阳性（PC）和阴性对照（NC）。仅氩气流也用作气体流量控制（F）。使用集落形成单位（CFU）回收和共聚焦激光扫描显微镜（CLSM）来分析生物膜活力。结果表明，LTP 治疗开始后 30s，所有处理样本中多物种生物膜的活力均显著降低了 2 个以上对数（p<0.0001）。对于单物种生物膜，干酪乳杆菌与 PC 和 NC 相比，所有暴露时间的活菌数均显著降低了 1 个以上对数（p<0.0001）。对于白色念珠菌生物膜，与 PC 和 NC 相比，LTP 处理导致应用 60 和 120s 时细菌计数显著减少（分别为 1.55 和 1.90log10CFU/mL）（p<0.0001）。与 F 相比，LTP 在单物种生物膜中观察到的显著效果（p≤0.05）始于 LTP 应用的 60s，表明 LTP 对白色念珠菌和干酪乳杆菌单物种生物膜的时间依赖性影响。LTP 是一种潜在的治疗龋齿机制，作为一种有效的抗生物膜治疗方法，可用于治疗单物种和多物种致龋生物膜。
