Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India.
AMR & Nanotherapeutics Lab, Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India.
Biomed Phys Eng Express. 2024 Oct 8;10(6). doi: 10.1088/2057-1976/ad7f59.
Multi-drug resistance (MDR) infections are a significant global challenge, necessitating innovative and eco-friendly approaches for developing effective antimicrobial agents. This study focuses on the synthesis, characterization, and evaluation of cerium oxide nanoparticles (CeONPs) for their antioxidant, anti-inflammatory, and antibacterial properties. The CeONPs were synthesized using aaqueous extract through an environmentally friendly process. Characterization techniques included UV-visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), x-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive x-ray (EDX) analysis. The UV-vis spectroscopy shows the presence of peak at 320 nm which confirms the formation of CeONPs. The FT-IR analysis of the CeONPs revealed several distinct functional groups, with peak values at 3287, 2920, 2340, 1640, 1538, 1066, 714, and 574 cm. These peaks correspond to specific functional groups, including C-H stretching in alkynes and alkanes, C=C=O, C=C, alkanes, C-O-C, C-Cl, and C-Br, indicating the presence of diverse chemical bonds within the CeONPs. XRD revealed that the nanoparticles were highly crystalline with a face-centered cubic structure, and SEM images showed irregularly shaped, agglomerated particles ranging from 100-150 nm. In terms of biological activity, the synthesized CeONPs demonstrated significant antioxidant and anti-inflammatory properties. The nanoparticles exhibited 82.54% antioxidant activity at 100 μg ml, closely matching the 83.1% activity of ascorbic acid. Additionally, the CeONPs showed 65.2% anti-inflammatory activity at the same concentration, compared to 70.1% for a standard drug. Antibacterial testing revealed that the CeONPs were particularly effective against multi-drug resistant strains, including,, and MRSA, with moderate activity against. These findings suggest that CeONPs synthesized viahave strong potential as antimicrobial agents in addressing MDR infections.
多药耐药（MDR）感染是一个全球性的重大挑战，因此需要创新和环保的方法来开发有效的抗菌剂。本研究专注于合成、表征和评估氧化铈纳米粒子（CeONPs）的抗氧化、抗炎和抗菌特性。CeONPs 通过环保工艺用水提物合成。表征技术包括紫外-可见光谱、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和能量色散 X 射线（EDX）分析。紫外可见光谱显示在 320nm 处有一个峰，证实了 CeONPs 的形成。CeONPs 的 FT-IR 分析显示了几个不同的官能团，峰值分别在 3287、2920、2340、1640、1538、1066、714 和 574cm。这些峰对应于特定的官能团，包括炔烃和烷烃中的 C-H 伸缩、C=C=O、C=C、烷烃、C-O-C、C-Cl 和 C-Br，表明 CeONPs 中存在多种化学键。XRD 表明纳米粒子具有高结晶度的面心立方结构，SEM 图像显示不规则形状、团聚的颗粒尺寸在 100-150nm 之间。在生物活性方面，合成的 CeONPs 表现出显著的抗氧化和抗炎特性。纳米粒子在 100μgml 时表现出 82.54%的抗氧化活性，与抗坏血酸的 83.1%活性非常接近。此外，CeONPs 在相同浓度下表现出 65.2%的抗炎活性，而标准药物为 70.1%。抗菌测试表明，CeONPs 对包括 、 、MRSA 和 在内的多药耐药菌株特别有效，对 具有中等活性。这些发现表明，通过该方法合成的 CeONPs 作为应对 MDR 感染的抗菌剂具有很大的潜力。
