Multi-drug resistance (MDR) infections are a significant global challenge, necessitating innovative and eco-friendly approaches for developing effective antimicrobial agents. This study focuses on the synthesis, characterization, and evaluation of cerium oxide nanoparticles (CeONPs) for their antioxidant, anti-inflammatory, and antibacterial properties. The CeONPs were synthesized using aaqueous extract through an environmentally friendly process. Characterization techniques included UV-visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), x-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive x-ray (EDX) analysis. The UV-vis spectroscopy shows the presence of peak at 320 nm which confirms the formation of CeONPs. The FT-IR analysis of the CeONPs revealed several distinct functional groups, with peak values at 3287, 2920, 2340, 1640, 1538, 1066, 714, and 574 cm. These peaks correspond to specific functional groups, including C-H stretching in alkynes and alkanes, C=C=O, C=C, alkanes, C-O-C, C-Cl, and C-Br, indicating the presence of diverse chemical bonds within the CeONPs. XRD revealed that the nanoparticles were highly crystalline with a face-centered cubic structure, and SEM images showed irregularly shaped, agglomerated particles ranging from 100-150 nm. In terms of biological activity, the synthesized CeONPs demonstrated significant antioxidant and anti-inflammatory properties. The nanoparticles exhibited 82.54% antioxidant activity at 100 μg ml, closely matching the 83.1% activity of ascorbic acid. Additionally, the CeONPs showed 65.2% anti-inflammatory activity at the same concentration, compared to 70.1% for a standard drug. Antibacterial testing revealed that the CeONPs were particularly effective against multi-drug resistant strains, including,, and MRSA, with moderate activity against. These findings suggest that CeONPs synthesized viahave strong potential as antimicrobial agents in addressing MDR infections.