Feng Shi, Wang Chunlin, Xu Zhaoyang, Dou Baozhu, Wang Xue, Yang Lina, Lu Baohui, Gao Jie
College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
State-Local Joint Engineering Research Center of Ginseng Breeding and Application, Changchun 130118, China.
Microorganisms. 2024 Aug 25;12(9):1761. doi: 10.3390/microorganisms12091761.
The objective of this study was to identify effective agents for the prevention and control of ginseng root rot disease caused by . The inhibitory effects of 16 chemical fungicides and 10 biocontrol agents (strains) on mycelial growth and sclerotium formation in were determined using a plate confrontation essay. The results showed that the best chemical agents for inhibiting the mycelial growth and sclerotium formation of were fluconazole and fludioxonil, while FS6 and (Kono) were the best biocontrol agents (strains). The results of field trials in 2022 and 2023 showed that the control effects of fluconazole and fludioxonil on ginseng root rot disease were 90.60-98.16%, and those of the biocontrol agents FS6 and (Kono) were 94.80-97.24%, respectively. Chemical agents produced abnormal and twisted mycelia, while the biocontrol agents increased mycelial branching, dilated the mycelium tip, and revealed an abnormal balloon. All of the fungicides decreased the ergosterol content, changed the cell membrane permeability, and increased the protein and nucleic acid permeability. These results suggest that these are potential agents for controlling ginseng root rot disease, and their biochemical mechanisms of chemical and biocontrol of this disease were demonstrated.
本研究的目的是确定预防和控制由……引起的人参根腐病的有效药剂。采用平板对峙试验测定了16种化学杀菌剂和10种生物防治剂（菌株）对……菌丝生长和菌核形成的抑制作用。结果表明，抑制……菌丝生长和菌核形成的最佳化学药剂是氟康唑和咯菌腈，而FS6和……（科诺）是最佳生物防治剂（菌株）。2022年和2023年的田间试验结果表明，氟康唑和咯菌腈对人参根腐病的防治效果为90.60 - 98.16%，生物防治剂FS6和……（科诺）的防治效果分别为94.80 - 97.24%。化学药剂使菌丝产生异常和扭曲，而生物防治剂增加了菌丝分支，使菌丝尖端扩张，并出现异常膨大。所有杀菌剂均降低了麦角甾醇含量，改变了细胞膜通透性，增加了蛋白质和核酸通透性。这些结果表明，这些是控制人参根腐病的潜在药剂，并阐明了它们对该病进行化学和生物防治的生化机制。
