Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles; Department of Masticatory Science, Faculty of Dentistry, Mahidol University.
Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles.
J Vis Exp. 2024 Sep 13(211). doi: 10.3791/67203.
Temporomandibular joint (TMJ) pain and osteoarthritis (OA) are common and debilitating disorders that impair patients' quality of life. The mechanisms driving diseases-related pain are poorly understood, and current treatments fail to provide effective and long-term therapeutic effects. Additionally, pain assessment in research, particularly orofacial pain, poses several challenges that complicate studies in both clinical and basic science settings. Therefore, we have established an inflammatory TMJ pain mouse model via intra-articular injection of CFA (Complete Freund's Adjuvant) and evaluated pain behaviors by bite force measurement and the von Frey filament test. Mice with CFA injection exhibited orofacial pain behaviors compared to PBS injection, including reduced bite force and head withdrawal threshold in the von Frey filament test. These methods are relatively easy to execute to have reproducible results and can be potentially extended to pain studies for other disease models related to TMJ disorders. Together, bite force, and the von Frey filament tests are reliable in measuring orofacial pain, as demonstrated in CFA injection-induced painful TMJOA mouse models.
颞下颌关节（TMJ）疼痛和骨关节炎（OA）是常见且使人衰弱的疾病，会降低患者的生活质量。导致疾病相关疼痛的机制尚未被充分理解，并且当前的治疗方法无法提供有效且长期的治疗效果。此外，在研究中，尤其是口腔颌面部疼痛的评估存在一些挑战，这些挑战使得临床和基础科学研究变得复杂。因此，我们通过关节内注射完全弗氏佐剂（CFA）建立了炎症性 TMJ 疼痛小鼠模型，并通过咬合力测量和von Frey 纤维试验评估疼痛行为。与 PBS 注射相比，接受 CFA 注射的小鼠表现出口颌面部疼痛行为，包括咬合力降低和 von Frey 纤维试验中的头部撤回阈值降低。这些方法相对容易执行，结果具有可重复性，并且可以潜在地扩展到与 TMJ 紊乱相关的其他疾病模型的疼痛研究中。综上所述，咬合力和 von Frey 纤维试验在测量口腔颌面部疼痛方面是可靠的，这在 CFA 注射诱导的 TMJOA 疼痛小鼠模型中得到了验证。
