Twery M J, Moss R L
Peptides. 1985 Jul-Aug;6(4):609-13. doi: 10.1016/0196-9781(85)90161-5.
The effects of iontophoretically applied human pancreatic growth hormone-releasing factor (hpGRF), peptide histidine isoleucine (PHI-27), and somatostatin (SS) on the extracellular activity of single cells in the hypothalamus, thalamus, and cortex of the rat brain were studied in urethane-anesthetized, male rats. Neurons with membrane sensitivity to hpGRF, PHI-27, and SS were present in each brain region. Although neurons excited by these peptides were encountered in thalamus and hypothalamus, depression of neuronal firing was the predominant response observed. Overall, the neurons responding to hpGRF also possessed membrane sensitivity to PHI-27, whereas, the hpGRF sensitive neurons appeared to be more divided as to their ability to respond to SS. The results clearly demonstrate that hpGRF and PHI-27 are capable of affecting the membrane excitability of neurons in several brain regions. The distribution of neurons sensitive to hpGRF suggests that hypothalamic GRF, in addition to its well documented role in the regulation of pituitary growth hormone secretion, may subserve other physiological events in the rat central nervous system as a neurotransmitter and/or neuromodulator.
在氨基甲酸乙酯麻醉的雄性大鼠中，研究了离子电渗法应用人胰腺生长激素释放因子（hpGRF）、肽组氨酸异亮氨酸（PHI - 27）和生长抑素（SS）对大鼠脑下丘脑、丘脑和皮质单细胞细胞外活性的影响。每个脑区均存在对hpGRF、PHI - 27和SS具有膜敏感性的神经元。虽然在丘脑和下丘脑中发现了被这些肽兴奋的神经元，但观察到的主要反应是神经元放电抑制。总体而言，对hpGRF有反应的神经元对PHI - 27也具有膜敏感性，而对hpGRF敏感的神经元对SS的反应能力似乎更具分化性。结果清楚地表明，hpGRF和PHI - 27能够影响多个脑区神经元的膜兴奋性。对hpGRF敏感的神经元分布表明，下丘脑GRF除了在调节垂体生长激素分泌中已被充分记录的作用外，可能作为神经递质和/或神经调节剂在大鼠中枢神经系统中参与其他生理活动。
