Whole-Genome Sequencing of the Entomopathogenic Fungus KMZW-1 and Its Efficacy Against .

KMZW-1 is recognized for its potential as a biocontrol agent against agricultural and forestry pests, particularly due to its compatibility with integrated pest management (IPM) strategies. This study aimed to investigate the complete genome of KMZW-1 and assess its pathogenicity against . Whole-genome sequencing revealed a genome size of 47,239,278 bp, comprising 27 contigs, with a GC content of 51.16% and fungus identified as KMZW-1. The genome completeness was assessed as 97.93% using BUSCO analysis, the DFVF sequence identifier was 0G092560.1, and AntiSMASH analysis identified 35 gene clusters associated with secondary metabolite biosynthesis, providing insights into the genetic basis of its pathogenic mechanisms and biocontrol potential. Comparative genomic analysis found 269 unique genes for KMZW-1, and collinearity analysis exhibited a high degree of synteny with . The pathogenicity of KMZW-1 was assessed using concentrations ranging from 1 × 10 to 1 × 10 conidia/mL. Higher concentrations (1 × 10 to 1 × 10 conidia/mL) resulted in significantly increased cumulative mortality rates of adults compared to the control group. Notably, the pathogenicity was higher in male adults than in females. Probit analysis yielded LC (50% lethal concentration) values of 5.662 for female and 4.486 for male adults. In summary, , KMZW-1 exhibits strong insecticidal activity against and shows potential as a biocontrol agent with IPM strategies. These findings provide robust genomic evidence supporting the use of KMZW-1 in managing against populations.